Uploaded October 2025 | Updated September 2026, 1 hour ago
At PacBio PRISM, Dr. Josh Clevenger of HudsonAlpha Institute for Biotechnology discusses how scaled, low-coverage HiFi sequencing is enhancing agricultural genomics. Learn how this approach enables the detection of previously hidden structural variants that are crucial for accelerating crop improvement, making the power of long-read data more accessible for population-level studies.
Dr. Clevenger explains how conventional genotyping methods often fail to identify complex variations, such as the copy number variant responsible for critical disease resistance in peanuts. By applying long-read sequencing even at low coverage (1–4x), breeding programs can gain a more complete picture of genetic variation. This presentation details the workflows that make large-scale HiFi genotyping feasible, from high-throughput DNA extraction and shearing to multiplexed library preparation.
Discover the tangible benefits through case studies in peanut, blueberry, and hemp. The data shows that analyzing low-pass HiFi reads on a pangenome graph yields exponentially more variants compared to short-read data at the same coverage. This increased resolution helps locate high-effect QTLs for traits like fruiting time in blueberries and clarifies the complex genetics of sex determination in hemp, advancing the development of more resilient and productive crops.
00:00 Introduction: the impact of HiFi in genomics
01:00 Applying translational genomics for food security
02:44 The need for scaled long-read sequencing
04:56 Making HiFi accessible: the Veil Genomics mission
05:58 Pangenomes reveal previously hidden traits
07:17 Workflow for high-throughput HiFi genotyping
09:07 Is low-coverage HiFi better? A peanut breeding study
10:23 Optimal sequencing coverage & variant discovery
12:47 Case study: uncovering disease resistance in peanut
15:20 Visualizing read coverage: HiFi vs. short reads
16:39 Case study: mapping fruiting time QTL in blueberry
18:49 Case study: decoding the complex hemp Y chromosome
21:02 The future of scaled agricultural genomics
Subscribe for more insights from PacBio: youtube.com/@PacificBiosciences?sub_confirmation=1
Learn more about PacBio at pacb.com
Legal & Trademarks: Visit pacb.com/legal-and-trademarks
At PacBio PRISM, Dr. Josh Clevenger of HudsonAlpha Institute for Biotechnology discusses how scaled, low-coverage HiFi sequencing is enhancing agricultural genomics. Learn how this approach enables the detection of previously hidden structural variants that are crucial for accelerating crop improvement, making the power of long-read data more accessible for population-level studies.
Dr. Clevenger explains how conventional genotyping methods often fail to identify complex variations, such as the copy number variant responsible for critical disease resistance in peanuts. By applying long-read sequencing even at low coverage (1–4x), breeding programs can gain a more complete picture of genetic variation. This presentation details the workflows that make large-scale HiFi genotyping feasible, from high-throughput DNA extraction and shearing to multiplexed library preparation.
Discover the tangible benefits through case studies in peanut, blueberry, and hemp. The data shows that analyzing low-pass HiFi reads on a pangenome graph yields exponentially more variants compared to short-read data at the same coverage. This increased resolution helps locate high-effect QTLs for traits like fruiting time in blueberries and clarifies the complex genetics of sex determination in hemp, advancing the development of more resilient and productive crops.
00:00 Introduction: the impact of HiFi in genomics
01:00 Applying translational genomics for food security
02:44 The need for scaled long-read sequencing
04:56 Making HiFi accessible: the Veil Genomics mission
05:58 Pangenomes reveal previously hidden traits
07:17 Workflow for high-throughput HiFi genotyping
09:07 Is low-coverage HiFi better? A peanut breeding study
10:23 Optimal sequencing coverage & variant discovery
12:47 Case study: uncovering disease resistance in peanut
15:20 Visualizing read coverage: HiFi vs. short reads
16:39 Case study: mapping fruiting time QTL in blueberry
18:49 Case study: decoding the complex hemp Y chromosome
21:02 The future of scaled agricultural genomics
Subscribe for more insights from PacBio: youtube.com/@PacificBiosciences?sub_confirmation=1
Learn more about PacBio at pacb.com
Legal & Trademarks: Visit pacb.com/legal-and-trademarks
