Uploaded April 2026 | Updated September 2026, 1 day ago
SPRQ-Nx chemistry reduces HiFi genome costs by 30 to 40% by enabling SMRT Cell reuse on the Revio system -- with no changes to library prep, no hardware upgrades, and consistent variant calling performance across uses.
In this webinar, PacBio product manager Matthew Boitano introduces SPRQ-Nx multiuse SMRT Cell chemistry, including expanded 5hmC methylation detection alongside 5mC and 6mA. Two beta site speakers share real-world results: Margaret Gibson from Children's Mercy Hospital, where HiFi long-read sequencing has increased diagnostic findings by 10% over standard of care in pediatric rare disease, and Katherine Munson from the University of Washington Long Read Sequencing Center, where SPRQ-Nx delivered equivalent or higher yield and quality scores for HiFi and Fiber-seq libraries, with similar performance using Kinnex kits.
*What you'll learn*
- Evaluate how SPRQ-Nx multiuse SMRT Cells lower per-genome costs by 30 to 40% with the same library prep and instruments
- Compare SPRQ-Nx beta performance data across HiFi, Fiber-seq, and Kinnex library types from 2 independent sites
- Assess the impact of HiFi long-read sequencing, including 37% diagnostic findings versus 26% for standard of care testing
- Understand how 5hmC detection expands multiomic capabilities at no additional cost or library prep steps
*Featured speakers*
- Matthew Boitano, Sr. Staff Product Manager (PacBio)
- Margaret Gibson, MBA, MB(ASCP)CM, Clinical Laboratory Manager (Children's Mercy Hospital)
- Katherine M. Munson, Research Scientist/Engineer (University of Washington)
*Chapters*
00:00 Introduction
01:49 SPRQ-Nx overview and chemistry
04:31 Multiuse SMRT Cell workflow
07:06 Pre-development HG002 data and assemblies
08:17 5hmC detection capability
09:21 Beta program performance
10:43 Margaret Gibson -- Clinical genomics at Children's Mercy
15:17 PacBio advantages over conventional testing
15:57 Potential pre-clinical implementation timeline
17:50 Diagnostic findings: HiFi vs standard of care
19:32 Challenges in long-read sequencing adoption
20:05 SPRQ-Nx impact for labs
22:23 Future plans at Children's Mercy
23:32 Katy Munson -- UW Long Read Sequencing Center
24:56 Research projects and sequencing consortia
26:56 Fiber-seq chromatin accessibility assay
29:00 SPRQ-Nx beta experimental design
31:21 Performance comparison: SPRQ vs SPRQ-Nx
32:24 Sequencing volume and cost results
33:27 Analysis plans: assembly and variant calling
35:10 Fiber-seq tagging comparison across chemistries
36:32 Summary of findings
37:43 Acknowledgments
38:10 Q&A session
*Resources*
- Learn more about the Revio system and SPRQ-Nx: pacb.com/revio
- Download SPRQ-Nx datasets (HG002 and more): pacb.com/datasets
- Explore PacBio upcoming events and webinars: pacb.com/events
- PacBio methylation detection (5mC, 5hmC, 6mA): pacb.com/technology/hifi-sequencing/epigenetics
What is the most impactful application of lower-cost HiFi genomes for your lab or research program? *Comment below.*
*Subscribe for more PacBio genomics content:*
youtube.com/@PacificBiosciences?sub_confirmation=1
Legal & Trademarks: pacb.com/legal-and-trademarks
#HiFiSequencing #SPRQ #PacBio
SPRQ-Nx chemistry reduces HiFi genome costs by 30 to 40% by enabling SMRT Cell reuse on the Revio system -- with no changes to library prep, no hardware upgrades, and consistent variant calling performance across uses.
In this webinar, PacBio product manager Matthew Boitano introduces SPRQ-Nx multiuse SMRT Cell chemistry, including expanded 5hmC methylation detection alongside 5mC and 6mA. Two beta site speakers share real-world results: Margaret Gibson from Children's Mercy Hospital, where HiFi long-read sequencing has increased diagnostic findings by 10% over standard of care in pediatric rare disease, and Katherine Munson from the University of Washington Long Read Sequencing Center, where SPRQ-Nx delivered equivalent or higher yield and quality scores for HiFi and Fiber-seq libraries, with similar performance using Kinnex kits.
*What you'll learn*
- Evaluate how SPRQ-Nx multiuse SMRT Cells lower per-genome costs by 30 to 40% with the same library prep and instruments
- Compare SPRQ-Nx beta performance data across HiFi, Fiber-seq, and Kinnex library types from 2 independent sites
- Assess the impact of HiFi long-read sequencing, including 37% diagnostic findings versus 26% for standard of care testing
- Understand how 5hmC detection expands multiomic capabilities at no additional cost or library prep steps
*Featured speakers*
- Matthew Boitano, Sr. Staff Product Manager (PacBio)
- Margaret Gibson, MBA, MB(ASCP)CM, Clinical Laboratory Manager (Children's Mercy Hospital)
- Katherine M. Munson, Research Scientist/Engineer (University of Washington)
*Chapters*
00:00 Introduction
01:49 SPRQ-Nx overview and chemistry
04:31 Multiuse SMRT Cell workflow
07:06 Pre-development HG002 data and assemblies
08:17 5hmC detection capability
09:21 Beta program performance
10:43 Margaret Gibson -- Clinical genomics at Children's Mercy
15:17 PacBio advantages over conventional testing
15:57 Potential pre-clinical implementation timeline
17:50 Diagnostic findings: HiFi vs standard of care
19:32 Challenges in long-read sequencing adoption
20:05 SPRQ-Nx impact for labs
22:23 Future plans at Children's Mercy
23:32 Katy Munson -- UW Long Read Sequencing Center
24:56 Research projects and sequencing consortia
26:56 Fiber-seq chromatin accessibility assay
29:00 SPRQ-Nx beta experimental design
31:21 Performance comparison: SPRQ vs SPRQ-Nx
32:24 Sequencing volume and cost results
33:27 Analysis plans: assembly and variant calling
35:10 Fiber-seq tagging comparison across chemistries
36:32 Summary of findings
37:43 Acknowledgments
38:10 Q&A session
*Resources*
- Learn more about the Revio system and SPRQ-Nx: pacb.com/revio
- Download SPRQ-Nx datasets (HG002 and more): pacb.com/datasets
- Explore PacBio upcoming events and webinars: pacb.com/events
- PacBio methylation detection (5mC, 5hmC, 6mA): pacb.com/technology/hifi-sequencing/epigenetics
What is the most impactful application of lower-cost HiFi genomes for your lab or research program? *Comment below.*
*Subscribe for more PacBio genomics content:*
youtube.com/@PacificBiosciences?sub_confirmation=1
Legal & Trademarks: pacb.com/legal-and-trademarks
#HiFiSequencing #SPRQ #PacBio
