Uploaded January 2026 | Updated September 2026, 37 minutes ago
“QC, QC, QC.” For Shana McDevitt, Director of Sequencing at UC Berkeley, this is the most crucial piece of advice for any core lab diving into long-read sequencing. In this installment of the PacBio SPRQ Your Research series, she shares her hands-on experience and hard-won wisdom on why uncompromising sample quality control is the foundation of exceptional HiFi sequencing results.
Discover why meticulous QC is non-negotiable, especially when working with challenging and exotic samples—from insect legs and lizard tails to coral sperm. Learn how the SPRQ reagent kits for the Revio system transformed her lab’s capabilities, enabling high-quality sequencing from low-input samples that were previously impossible to process. McDevitt also discusses the expanding applications of HiFi sequencing, including T2T genomics, metagenomics, and the exciting potential of calling 6mA methylation data.
00:00 Introduction: A Core director's perspective
00:28 The #1 rule for PacBio sequencing success
02:12 The challenge: high-quality DNA from exotic samples
03:47 How SPRQ kits unlocked low-input sequencing
07:14 Sequencing unconventional species
10:12 Expanding research with 6mA methylation data
Subscribe for more insights from PacBio: youtube.com/@PacificBiosciences?sub_confirmation=1
Learn more about PacBio at pacb.com
Legal & Trademarks: Visit pacb.com/legal-and-trademarks
“QC, QC, QC.” For Shana McDevitt, Director of Sequencing at UC Berkeley, this is the most crucial piece of advice for any core lab diving into long-read sequencing. In this installment of the PacBio SPRQ Your Research series, she shares her hands-on experience and hard-won wisdom on why uncompromising sample quality control is the foundation of exceptional HiFi sequencing results.
Discover why meticulous QC is non-negotiable, especially when working with challenging and exotic samples—from insect legs and lizard tails to coral sperm. Learn how the SPRQ reagent kits for the Revio system transformed her lab’s capabilities, enabling high-quality sequencing from low-input samples that were previously impossible to process. McDevitt also discusses the expanding applications of HiFi sequencing, including T2T genomics, metagenomics, and the exciting potential of calling 6mA methylation data.
00:00 Introduction: A Core director's perspective
00:28 The #1 rule for PacBio sequencing success
02:12 The challenge: high-quality DNA from exotic samples
03:47 How SPRQ kits unlocked low-input sequencing
07:14 Sequencing unconventional species
10:12 Expanding research with 6mA methylation data
Subscribe for more insights from PacBio: youtube.com/@PacificBiosciences?sub_confirmation=1
Learn more about PacBio at pacb.com
Legal & Trademarks: Visit pacb.com/legal-and-trademarks
