Uploaded January 2026 | Updated September 2026, 1 hour ago
*Learn how PacBio HiFi long-read sequencing delivers greater than 99.9% accuracy—without PCR amplification.*
By combining multiple passes over the same native molecule, HiFi sequencing generates a single highly accurate long read. This process overcomes GC bias, resolves hard-to-sequence regions, and detects methylation—all in a single run. The result is an accurate, long-read sequence and epigenetic data you can trust.
*The HiFi difference*
- *Unmatched accuracy:* Achieve 99.9% accuracy to confidently detect variants of all types.
- *Uniform coverage:* Sequence GC-rich, repetitive, and "dark" regions that other technologies miss.
- *Native methylation:* Detect base modifications directly without PCR or bisulfite treatment.
- *Long read lengths:* Span full-lengths to resolve structural variants and isoforms.
00:00 Introduction to HiFi long read sequencing
00:27 SMRTbell library preparation
00:54 Sequencing on the SMRT Cell
01:22 Native DNA methylation detection
01:57 Multiomic data analysis
02:22 Applications and market segments
*Resources*
pacb.com/blog/long-read-sequencing
pacb.com/technology/hifi-sequencing
How would simultaneous epigenetic data impact your current genomic research? *Comment below.*
*Subscribe for more PacBio genomics content:*
youtube.com/@PacificBiosciences?sub_confirmation=1
Learn more about PacBio at pacb.com
Legal & Trademarks: pacb.com/legal-and-trademarks
#PacBio #HiFiSequencing #Genomics
*Learn how PacBio HiFi long-read sequencing delivers greater than 99.9% accuracy—without PCR amplification.*
By combining multiple passes over the same native molecule, HiFi sequencing generates a single highly accurate long read. This process overcomes GC bias, resolves hard-to-sequence regions, and detects methylation—all in a single run. The result is an accurate, long-read sequence and epigenetic data you can trust.
*The HiFi difference*
- *Unmatched accuracy:* Achieve 99.9% accuracy to confidently detect variants of all types.
- *Uniform coverage:* Sequence GC-rich, repetitive, and "dark" regions that other technologies miss.
- *Native methylation:* Detect base modifications directly without PCR or bisulfite treatment.
- *Long read lengths:* Span full-lengths to resolve structural variants and isoforms.
00:00 Introduction to HiFi long read sequencing
00:27 SMRTbell library preparation
00:54 Sequencing on the SMRT Cell
01:22 Native DNA methylation detection
01:57 Multiomic data analysis
02:22 Applications and market segments
*Resources*
pacb.com/blog/long-read-sequencing
pacb.com/technology/hifi-sequencing
How would simultaneous epigenetic data impact your current genomic research? *Comment below.*
*Subscribe for more PacBio genomics content:*
youtube.com/@PacificBiosciences?sub_confirmation=1
Learn more about PacBio at pacb.com
Legal & Trademarks: pacb.com/legal-and-trademarks
#PacBio #HiFiSequencing #Genomics
