Uploaded October 2025 | Updated September 2026, 13 hours ago
*How does HiFi long-read sequencing provide a clearer view of the human microbiome?* In this webinar, see how HiFi reads deliver the accuracy and read length needed to resolve species and strains, and recover complete or near-complete MAGs from complex, high-host samples.
PacBio HiFi sequencing generates highly accurate long reads from single DNA molecules. This enables the assembly of high-quality, often complete, metagenome-assembled genomes (MAGs). It also allows for full-length 16S rRNA gene sequencing for high-resolution taxonomic profiling.
*What you’ll learn*
* Explore microbial genomics applications for HiFi sequencing
* Use full-length 16S to investigate disease-associated microbes
* Assemble high-quality MAGs from metagenomic samples
* Compare long-read technologies for microbiome research
00:00 Welcome & introductions
02:23 What is PacBio HiFi sequencing?
04:48 Key applications in microbial genomics
07:54 Throughput and cost estimates per sample
10:11 The challenge of recurrent urinary tract infections (A. Earl)
12:30 Study design: Tracking the uropathogen reservoir
15:23 Using full-length 16S sequencing for high-host samples
19:40 Amplicon sequence variants (ASVs) reveal rUTI associations
22:45 Integrating plate sweep metagenomics for strain-level insight
27:32 The microbiome’s role in childhood undernutrition (J. Minich)
30:22 Comparing long-read platforms for metagenome assembly
35:46 Why accuracy matters: Cost and quality per complete genome
42:53 Pangenome analysis and genome stability over time
48:00 Live Q&A begins
What microbiome research question would you tackle with HiFi sequencing? *Comment below.*
*Subscribe for more PacBio genomics content:*
youtube.com/@PacificBiosciences?sub_confirmation=1
Learn more about PacBio at pacb.com
Legal & Trademarks: pacb.com/legal-and-trademarks
#Microbiome #Metagenomics #HiFiSequencing
*How does HiFi long-read sequencing provide a clearer view of the human microbiome?* In this webinar, see how HiFi reads deliver the accuracy and read length needed to resolve species and strains, and recover complete or near-complete MAGs from complex, high-host samples.
PacBio HiFi sequencing generates highly accurate long reads from single DNA molecules. This enables the assembly of high-quality, often complete, metagenome-assembled genomes (MAGs). It also allows for full-length 16S rRNA gene sequencing for high-resolution taxonomic profiling.
*What you’ll learn*
* Explore microbial genomics applications for HiFi sequencing
* Use full-length 16S to investigate disease-associated microbes
* Assemble high-quality MAGs from metagenomic samples
* Compare long-read technologies for microbiome research
00:00 Welcome & introductions
02:23 What is PacBio HiFi sequencing?
04:48 Key applications in microbial genomics
07:54 Throughput and cost estimates per sample
10:11 The challenge of recurrent urinary tract infections (A. Earl)
12:30 Study design: Tracking the uropathogen reservoir
15:23 Using full-length 16S sequencing for high-host samples
19:40 Amplicon sequence variants (ASVs) reveal rUTI associations
22:45 Integrating plate sweep metagenomics for strain-level insight
27:32 The microbiome’s role in childhood undernutrition (J. Minich)
30:22 Comparing long-read platforms for metagenome assembly
35:46 Why accuracy matters: Cost and quality per complete genome
42:53 Pangenome analysis and genome stability over time
48:00 Live Q&A begins
What microbiome research question would you tackle with HiFi sequencing? *Comment below.*
*Subscribe for more PacBio genomics content:*
youtube.com/@PacificBiosciences?sub_confirmation=1
Learn more about PacBio at pacb.com
Legal & Trademarks: pacb.com/legal-and-trademarks
#Microbiome #Metagenomics #HiFiSequencing
