Uploaded July 2026 | Updated September 2026, 41 minutes ago
For human microbiome research, this webinar compares full-length 16S and shotgun metagenomics for strain-level resolution, cMAG recovery, and host-rich samples.
In this PacBio webinar, Jeremy Wilkinson, PhD, introduces HiFi long-read sequencing for microbial genomics. Ashlee M. Earl, PhD, and Jeremiah Minich, PhD, then share human microbiome examples spanning Kinnex full-length 16S, ASV-level analysis, complete MAG recovery, custom genome databases, and live Q&A.
*Key takeaways*
- PacBio HiFi sequencing supports full-length 16S, ITS, shotgun metagenomics, metagenome assembly, microbial WGS, and viral sequencing workflows.
- Kinnex full-length 16S helped move one recurrent UTI microbiome analysis from more than 300 species to more than 8,000 ASVs.
- In one long-read metagenomics comparison, Revio generated 2.3x more cMAGs than ONT.
- Custom genome databases increased sequence classification to 82% in Jeremiah Minich's human microbiome dataset.
- The Q&A covers 16S depth, shotgun metagenomics, host depletion, QIAGEN and Zymo kits, adaptive sampling, and workflow selection.
*Featured speakers*
- Jeremy Wilkinson, PhD (PacBio)
- Ashlee M. Earl, PhD (Broad Institute of MIT & Harvard)
- Jeremiah Minich, PhD (Baylor University)
*Section summaries*
00:00 - *Webinar setup.* Jeremy Wilkinson frames the session as a rebroadcast on PacBio HiFi sequencing for human microbiome research, followed by live Q&A.
03:34 - *HiFi microbial genomics overview.* The introduction explains how long, accurate HiFi reads support full-length marker sequencing, shotgun profiling, metagenome assembly, microbial WGS, and viral sequencing.
11:18 - *Kinnex and recurrent UTI microbiomes.* Ashlee Earl discusses host-rich vaginal microbiome samples, Kinnex full-length 16S, ASV-level analysis, and rUTI-associated microbial ecology.
29:20 - *Culture-independent meta-pan genomics.* Jeremiah Minich compares PacBio Revio, ONT, and Illumina synthetic long reads for complete MAG recovery and downstream classification.
49:06 - *Live Q&A.* Jeremy answers workflow questions about databases, 16S target genes, shotgun metagenomics, host depletion, adaptive sampling, and sample prep.
*Chapters*
00:00 Welcome and webinar setup
02:46 Why revisit this microbiome session now
03:34 HiFi sequencing for microbial genomics
07:00 Full-length 16S and shotgun metagenomics workflows
09:10 Vega, Revio, Kinnex, and cost-throughput considerations
11:18 Kinnex in recurrent urinary tract microbiomes
16:33 Host-rich samples and full-length 16S RNA genes
20:54 From species calls to ASV-level resolution
26:40 Kinnex takeaways and companion culture data
29:20 Culture-independent meta-pan genomics
32:14 PacBio, ONT, and Illumina comparison design
35:07 Complete MAG benchmarks and Revio results
40:34 Custom databases and classification rates
42:05 LongPlex validation with Revio and Vega
49:06 Live Q&A begins
52:29 16S, shotgun metagenomics, and target-gene questions
55:15 Sample prep, host depletion, and adaptive sampling
58:08 Closing remarks
*Resources*
- Webinar landing page: programs.pacb.com/l/1652/2026-05-13/45j2fh
- PacBio metagenomics datasets: github.com/PacificBiosciences/pb-metagenomics-tools/blob/master/docs/PacBio-Data.md
- PacBio datasets: pacb.com/datasets
Where is the bigger bottleneck in your human microbiome workflow: strain-level resolution, host DNA, or functional annotation? *Comment below.*
*Subscribe for more PacBio genomics content:*
youtube.com/@PacificBiosciences?sub_confirmation=1
Learn more about PacBio at pacb.com
Legal & Trademarks: pacb.com/legal-and-trademarks
#Microbiome #Metagenomics #PacBio
For human microbiome research, this webinar compares full-length 16S and shotgun metagenomics for strain-level resolution, cMAG recovery, and host-rich samples.
In this PacBio webinar, Jeremy Wilkinson, PhD, introduces HiFi long-read sequencing for microbial genomics. Ashlee M. Earl, PhD, and Jeremiah Minich, PhD, then share human microbiome examples spanning Kinnex full-length 16S, ASV-level analysis, complete MAG recovery, custom genome databases, and live Q&A.
*Key takeaways*
- PacBio HiFi sequencing supports full-length 16S, ITS, shotgun metagenomics, metagenome assembly, microbial WGS, and viral sequencing workflows.
- Kinnex full-length 16S helped move one recurrent UTI microbiome analysis from more than 300 species to more than 8,000 ASVs.
- In one long-read metagenomics comparison, Revio generated 2.3x more cMAGs than ONT.
- Custom genome databases increased sequence classification to 82% in Jeremiah Minich's human microbiome dataset.
- The Q&A covers 16S depth, shotgun metagenomics, host depletion, QIAGEN and Zymo kits, adaptive sampling, and workflow selection.
*Featured speakers*
- Jeremy Wilkinson, PhD (PacBio)
- Ashlee M. Earl, PhD (Broad Institute of MIT & Harvard)
- Jeremiah Minich, PhD (Baylor University)
*Section summaries*
00:00 - *Webinar setup.* Jeremy Wilkinson frames the session as a rebroadcast on PacBio HiFi sequencing for human microbiome research, followed by live Q&A.
03:34 - *HiFi microbial genomics overview.* The introduction explains how long, accurate HiFi reads support full-length marker sequencing, shotgun profiling, metagenome assembly, microbial WGS, and viral sequencing.
11:18 - *Kinnex and recurrent UTI microbiomes.* Ashlee Earl discusses host-rich vaginal microbiome samples, Kinnex full-length 16S, ASV-level analysis, and rUTI-associated microbial ecology.
29:20 - *Culture-independent meta-pan genomics.* Jeremiah Minich compares PacBio Revio, ONT, and Illumina synthetic long reads for complete MAG recovery and downstream classification.
49:06 - *Live Q&A.* Jeremy answers workflow questions about databases, 16S target genes, shotgun metagenomics, host depletion, adaptive sampling, and sample prep.
*Chapters*
00:00 Welcome and webinar setup
02:46 Why revisit this microbiome session now
03:34 HiFi sequencing for microbial genomics
07:00 Full-length 16S and shotgun metagenomics workflows
09:10 Vega, Revio, Kinnex, and cost-throughput considerations
11:18 Kinnex in recurrent urinary tract microbiomes
16:33 Host-rich samples and full-length 16S RNA genes
20:54 From species calls to ASV-level resolution
26:40 Kinnex takeaways and companion culture data
29:20 Culture-independent meta-pan genomics
32:14 PacBio, ONT, and Illumina comparison design
35:07 Complete MAG benchmarks and Revio results
40:34 Custom databases and classification rates
42:05 LongPlex validation with Revio and Vega
49:06 Live Q&A begins
52:29 16S, shotgun metagenomics, and target-gene questions
55:15 Sample prep, host depletion, and adaptive sampling
58:08 Closing remarks
*Resources*
- Webinar landing page: programs.pacb.com/l/1652/2026-05-13/45j2fh
- PacBio metagenomics datasets: github.com/PacificBiosciences/pb-metagenomics-tools/blob/master/docs/PacBio-Data.md
- PacBio datasets: pacb.com/datasets
Where is the bigger bottleneck in your human microbiome workflow: strain-level resolution, host DNA, or functional annotation? *Comment below.*
*Subscribe for more PacBio genomics content:*
youtube.com/@PacificBiosciences?sub_confirmation=1
Learn more about PacBio at pacb.com
Legal & Trademarks: pacb.com/legal-and-trademarks
#Microbiome #Metagenomics #PacBio
