Difference between Barfoed and Benedict test @quickbiochemistrybasics
Difference between Barfoed and Benedict test  @quickbiochemistrybasics
Uploaded May 2019 | Updated September 2026, 2 weeks ago
Barfoed test is used to diffrentiate monosaccharides vs disaccharides, while benedict test is used to detect reducing monosaccharide like glucose (in urine as indication of diabetes). both test uses solution of copper sulphate, however barfoed test is done in acidic conditions while benedict test is done in alkaline conditions.
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Difference between Barfoed and Benedict test

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