Quick Biochemistry Basics | Fluorescence recovery after photobleaching | FRAP @quickbiochemistrybasics | Uploaded 5 years ago | Updated 12 hours ago
Fluorescence recovery after photobleaching (FRAP) is a technique for determining the kinetics of diffusion of molecules through tissue or cells. It is capable of quantifying the two dimensional lateral diffusion of a molecularly thin film containing fluorescently labeled probes, or to examine single cells.

$Resolution of a Microscope This video is about, how diffraction limits ability of light microscope to resolve small objects. Resolution is the ability of an optical instrument/microscope to distinguish between two closely spaced objects (governed by Rayleigh criterion) and ability of optical instrument/microscope to see tiny object clearly. The resolution of microscope is mathematically given by Abbe diffraction limit, that depends on the wavelength of light used and Numerical Aperture (NA). The resolution of optical microscope depends on the diffraction, while resolution of super resolution microscope is independent of diffraction limitation.$

Column chromatography and discovery of DNA polymerase

Succinate dehydrogenase (complex 2 of ETC)

Fluorescence recovery after photobleaching | FRAP @quickbiochemistrybasics