Uploaded August 2025 | Updated September 2026, 2 weeks ago
Writing Ionic Formulas
Dr. DeBacco
1. Identify the Ions
Determine the cation (positive ion) and anion (negative ion).
Example: Sodium (Na⁺) and Chloride (Cl⁻)
2. Determine the Charges
Use the periodic table or ion chart to find the typical charges.
Example: Calcium (Ca²⁺) and Nitrate (NO₃⁻)
3. Balance the Charges
Total positive charge must balance total negative charge.
You may need multiple ions to achieve balance.
Example: Ca²⁺ + 2(NO₃⁻) → Ca(NO₃)₂
4. Write the Formula
Start with the cation, then the anion.
Use subscripts to show how many ions are needed.
Don’t show charges in the final formula—just the correct ratio.
Example: Aluminum (Al³⁺) and Oxygen (O²⁻) → Al₂O₃
Tips
Transition metals might have multiple charges Roman numerals can help
Fe²⁺ = iron(II)
Polyatomic ions like SO₄²⁻ or NH₄⁺ should be kept in parentheses if you need more than one.
Example: Mg²⁺ and SO₄²⁻ → MgSO₄
But Mg²⁺ and two NO₃⁻ → Mg(NO₃)₂
Link to Lecture Slides: drive.google.com/file/d/1hfpSqcfTnEa7yziX1-0bQbDQnu6Ngubj/view?usp=drive_link
*Due to the description character limit the full work cited for "Writing Ionic Formulas" can be viewed at... docs.google.com/document/d/1Ma3vDciR70ybAiAD_q_gjprwI1zwY8Qy/edit?usp=drive_link&ouid=104237452697237972847&rtpof=true&sd=true
Writing Ionic Formulas
Dr. DeBacco
1. Identify the Ions
Determine the cation (positive ion) and anion (negative ion).
Example: Sodium (Na⁺) and Chloride (Cl⁻)
2. Determine the Charges
Use the periodic table or ion chart to find the typical charges.
Example: Calcium (Ca²⁺) and Nitrate (NO₃⁻)
3. Balance the Charges
Total positive charge must balance total negative charge.
You may need multiple ions to achieve balance.
Example: Ca²⁺ + 2(NO₃⁻) → Ca(NO₃)₂
4. Write the Formula
Start with the cation, then the anion.
Use subscripts to show how many ions are needed.
Don’t show charges in the final formula—just the correct ratio.
Example: Aluminum (Al³⁺) and Oxygen (O²⁻) → Al₂O₃
Tips
Transition metals might have multiple charges Roman numerals can help
Fe²⁺ = iron(II)
Polyatomic ions like SO₄²⁻ or NH₄⁺ should be kept in parentheses if you need more than one.
Example: Mg²⁺ and SO₄²⁻ → MgSO₄
But Mg²⁺ and two NO₃⁻ → Mg(NO₃)₂
Link to Lecture Slides: drive.google.com/file/d/1hfpSqcfTnEa7yziX1-0bQbDQnu6Ngubj/view?usp=drive_link
*Due to the description character limit the full work cited for "Writing Ionic Formulas" can be viewed at... docs.google.com/document/d/1Ma3vDciR70ybAiAD_q_gjprwI1zwY8Qy/edit?usp=drive_link&ouid=104237452697237972847&rtpof=true&sd=true




![How Can You Prevent Hop Latent Viroid (HLVd) From Spreading?
How Can You Prevent Hop Latent Viroid HLVd From Spreading?
Professor DeBacco
Additional Video Sources:
Medicinal Genomics. (2023a, June 8). Understanding and Managing HOP latent viroid in cannabis - Zamir Punja, PhD [Video]. YouTube. https://www.youtube.com/watch?v=xQTRWQuUCzY
Starts with Testing/Screening
Knowing what you have
How Can You Prevent HLVd from Spreading?
Strict and consistent tool sterilization
PPE for workers
Rigorous testing protocol
Repeat testing of Mother room
Plant Eradication
Removing plants that test positive and removing them, followed by continual testing can reduce the presence of HLVd in a growing operation.
Cleaning Tools and Surfaces
This should be done on a regular basis.
However the product you select as your “cleaner” may make the area look clean but many not have any impact on HLVd
Not Effective Methods
Alcohol
Alcohol + flame
Flaming tools
H2O2
Acetic Acid (Vinegar)
Ammonium cleaners (Ex. Lysol)
UV Light
UV-C exposure on leaves and roots for 5min. And viroid was still present
What Does Work… (On Tools and Surfaces)
Virkon S 2%
Household Bleach 10-20% for 30-60sec.
Common lab saying… 10% bleach for 10min.
Mix and use the same day.
Same Can Not Be Applied to Root (Plant) Tissue
Previous products and recommendations are for tools and surfaces and those effective in those situations are not effective at cleaning plant tissue.
UV-C Irradiation
The viroid is very stable in plant tissue.
This makes it very hard to “clean” plant material which is why it should be bagged and physically removed.
Sap is much more Difficult
The “best” method for sap containing viroids may be Nucleases
Link to Lecture Slides: https://drive.google.com/file/d/1bKoKL-iZDk7VL11g46zDLL8cEOAnxuZe/view?usp=drive_link
*Due to the description character limit the full work cited for How Can You Prevent Hop Latent Viroid (HLVd) From Spreading? can be viewed at... https://docs.google.com/document/d/1va7Y3vADzL0X_HS82j2MRWTVNlHf_IFH/edit?usp=drive_link&ouid=104237452697237972847&rtpof=true&sd=true How Can You Prevent Hop Latent Viroid (HLVd) From Spreading?](https://i.ytimg.com/vi/ekSHtVF5eKo/mqdefault.jpg)




![Nitrogen Fertilizers Impact on CBD and THC Concentrations in Cannabis
Nitrogen Fertilizers Impact on CBD and THC Concentrations in Cannabis
Professor DeBacco
Research Article
Dilena, E., Close, D. C., Hunt, I., & Garland, S. M. (2023). Investigating how nitrogen nutrition and pruning impacts on CBD and THC concentration and plant biomass of Cannabis sativa. Scientific Reports, 13(1), 19533.
https://www.nature.com/articles/s41598-023-46369-5
Total Biomass and Total Cannabinoid Concentrations
Total biomass and total cannabinoid concentrations in % DW (on the y-axis) versus measured N concentrations in % DW (on each x-axis).
Treatment group labels are indicated by different colors.
The shape of the points (circle or triangle) indicates whether or not the plant associated with the data point was on the sun edge.
For each regression n = 20.
Inverse Relationship of [N] to [Cannabinoid]
The increase in biomass as N concentration increased from 3 to 6 was approximately 100%
Note the y-axis scale
However, the corresponding decrease in cannabinoid concentrations was approximately 67%
This entails a net decline in yield in terms of grams as N concentration increases
Three Key Points
1. Biomass was strongly and positively related to N
2. Cannabinoid concentrations were strongly and negatively related to N
3. Plants on the sun-edge tended to have higher biomass levels and cannabinoid concentrations
High Nitrogen Levels
Higher nitrogen nutrition raised the concentration of N in both inflorescence and leaf plant matter.
For very high levels of N nutrition (500 mg/mL), inflorescence cannabinoid concentrations decreased significantly (with little change in biomass) and leaf biomass increased significantly.
With higher N supply stem diameter also increased.
The net effect of increasing nitrogen nutrition on the total yield of cannabinoids was negative because the increase in biomass (which was only significant in leaves) was not enough to offset the consequent decrease in cannabinoid concentrations.
Take Home Message…
Cannabinoid concentration, as well as cannabinoid yield per plant were decreased with the increase in N supply.
Target Nitrogen Levels
Very high concentrations of fertilizer are not advisable because of lower cannabinoid concentration and yield, and that the optimal N nutrition is likely to be between 60 and 210 mg/L.
0.008-0.028 ounces per gallon
Prunings Impact
Double stem pruning was applied as an additional treatment to investigate efficacy on biomass increase.
Pruning treatment did not increase cannabinoid concentrations or affect biomass when measured at the final harvest.
Study Also Evaluate Sun Edge Plants
Across all treatments, as an artefact of this trial, sun-edge plants that were more directly exposed to sunlight showed a trend towards more biomass and higher cannabinoid concentrations
According to the statistical model developed for this study.
Self-shading of plants can limit the production of cannabinoids, so it is important to consider plant density and light intensity/spectrum.
*However, these results can only be considered preliminary and does warrants further research.
Link to Lecture Slides: https://drive.google.com/file/d/1lgV_46bDoXmPwXrAQAhfs7b04vcFISMU/view?usp=drive_link
*Due to the description character limit the full work cited for Nitrogen Fertilizers Impact on CBD and THC Concentrations in Cannabis can be viewed at... https://docs.google.com/document/d/1PAyFcsjILnKWvikPChNQmrt3hFAgH5Vh/edit?usp=drive_link&ouid=104237452697237972847&rtpof=true&sd=true Nitrogen Fertilizers Impact on CBD and THC Concentrations in Cannabis](https://i.ytimg.com/vi/gRAM9e2otLg/mqdefault.jpg)
