Uploaded February 2023 | Updated September 2026, 2 weeks ago
The Many Uses of Hydrogen Peroxide in the Garden
Professor DeBacco
What is Hydrogen Peroxide?
Simple molecule H2O2
Advantages of H2O2
Can kill both fungi and bacteria and algae
Copper also fits into this category but has many other issues
Ex. Interactions, phytotoxicity, residual, environmental contaminate
No residue left behind
Disadvantages of H2O2
Short residual time
Can require frequent applications
Contact kill only
Note: Not Sponsored by Any Company
All products were purchased at retail cost
No sponsorship of any products mentioned here
It is recommended that you purchase the product for your intended purpose and follow the printed label.
Agriculture Focused Hydrogen Peroxide Products
Biosafesystems product line
ZeroTol
Oxidate
Sanidate
Terraclean
Four Uses
Improved Seed Germination
Tool Sterilizer
Foliar Application
Soil Treatment
1. Improved Seed Germination
Research Article
Sorokin, A., Yadav, N. S., Gaudet, D., & Kovalchuk, I. (2021). Development and Standardization of Rapid and Efficient Seed Germination Protocol for Cannabis sativa. Bio-protocol, 11(1), e3875.
ncbi.nlm.nih.gov/pmc/articles/PMC7952943
Research Findings
Seed sterilization, germination, and seedlings development were carried out in an H2O2 solution of different concentrations
1% H2O2 solution showed the fastest and the most efficient germination.
High germination efficiency for very old cannabis seeds with lower viability was shown
2. Tool Sterilizer
Frequently cleaning tools can help prevent the grower being a vector for disease spread from one plant to another.
Tool Sterilizer Solution Make Fresh Daily
Once diluted the solution should be used for the day (or less).
If exposed to direct sunlight this will speed up the degradation process as well.
It will always look like clear water so be sure to put it in a special and labeled container.
At the end of the day a final submersion in the solution followed by a rise can help reduce the chance of corrosion of the equipment.
3. Foliar Application
This is one of the few bactericides that exist in general.
It also works on fungus as well
Foliar Application: Leaf and Soil Based Pathogens Controlled…
ZeroTol 2.0
Algae
Alternaria
Anthracnose
Aphanomyces
Bacterial spots and rots
Botrytis (grey mold)
Downy mildew
Erwinia
Foliar Application with No Residue
This reduces the chance of burring the plant
Be sure to follow the recommended concentration as with high concentrations there can be peroxide burn of the plant material.
Essentially, the products breaks down to water and oxygen gas.
Foliar Application Complete Coverage is Key
With this (and any other contact only product) good plant coverage is very important as this has a direct impact on the effectiveness of the product.
4. Soil Treatment
This is where hydrogen peroxide is showing greater interest and some initial results are the reason for its continued use.
Some areas have strict regulations on products for controlling soil based diseases.
This is for good reason as these can have long residuals and negatively impact non-target environments.
Soil Treatment
Hydrogen peroxide can control a wide range of soil based diseases with low residual so it will not have long term negative impacts on the environment.
It is much easier and cost effective compared to steam sterilization.
While this may be better it is not easy to do and is costly in both labor and equipment.
Soil Treatment Site Preparations
Clear crop residue
Some can be on the surface and/or partially incorporated into the soil, but a lot of plant residue will reduce soil penetration
The soil surface should be quick/rough tilled to allow for greater percolation into the soil profile.
Soil should have some moisture
Post Soil Treatment
Adding back in beneficial microbes
Manufacture says these can be added only 2-hours after the peroxide application.
This is when the foaming you may see on the soil surface during application has gone away.
Soil Treatment Micro Replenish TerraGrow
Following manufacture recommendations, TerraGrow was added to the same area that was treated with the TerraClean product 48-hours prior.
So, be sure to get the entire growing area when performing this treatment.
While it will not be 100% effective the delayed onset and mitigation of most pressure sure makes it more effective than many of the harsher chemical options that exist.
Cover crops are still an important component
For many soil based diseases you want reduce the chance of overwatering and excessive nitrogen fertilizers.
Link to Lecture Slides: drive.google.com/file/d/1AmVG6JHNzen6QqvS4RJkBuiYXq_ELBAQ/view?usp=share_link
*Due to the description character limit the full work cited for "The Many Uses of Hydrogen Peroxide in the Garden" can be viewed at... docs.google.com/document/d/1Ok6TYaGcwmiFpXwbZPg7PvD9ydidvm92/edit?usp=share_link&ouid=104237452697237972847&rtpof=true&sd=true
The Many Uses of Hydrogen Peroxide in the Garden
Professor DeBacco
What is Hydrogen Peroxide?
Simple molecule H2O2
Advantages of H2O2
Can kill both fungi and bacteria and algae
Copper also fits into this category but has many other issues
Ex. Interactions, phytotoxicity, residual, environmental contaminate
No residue left behind
Disadvantages of H2O2
Short residual time
Can require frequent applications
Contact kill only
Note: Not Sponsored by Any Company
All products were purchased at retail cost
No sponsorship of any products mentioned here
It is recommended that you purchase the product for your intended purpose and follow the printed label.
Agriculture Focused Hydrogen Peroxide Products
Biosafesystems product line
ZeroTol
Oxidate
Sanidate
Terraclean
Four Uses
Improved Seed Germination
Tool Sterilizer
Foliar Application
Soil Treatment
1. Improved Seed Germination
Research Article
Sorokin, A., Yadav, N. S., Gaudet, D., & Kovalchuk, I. (2021). Development and Standardization of Rapid and Efficient Seed Germination Protocol for Cannabis sativa. Bio-protocol, 11(1), e3875.
ncbi.nlm.nih.gov/pmc/articles/PMC7952943
Research Findings
Seed sterilization, germination, and seedlings development were carried out in an H2O2 solution of different concentrations
1% H2O2 solution showed the fastest and the most efficient germination.
High germination efficiency for very old cannabis seeds with lower viability was shown
2. Tool Sterilizer
Frequently cleaning tools can help prevent the grower being a vector for disease spread from one plant to another.
Tool Sterilizer Solution Make Fresh Daily
Once diluted the solution should be used for the day (or less).
If exposed to direct sunlight this will speed up the degradation process as well.
It will always look like clear water so be sure to put it in a special and labeled container.
At the end of the day a final submersion in the solution followed by a rise can help reduce the chance of corrosion of the equipment.
3. Foliar Application
This is one of the few bactericides that exist in general.
It also works on fungus as well
Foliar Application: Leaf and Soil Based Pathogens Controlled…
ZeroTol 2.0
Algae
Alternaria
Anthracnose
Aphanomyces
Bacterial spots and rots
Botrytis (grey mold)
Downy mildew
Erwinia
Foliar Application with No Residue
This reduces the chance of burring the plant
Be sure to follow the recommended concentration as with high concentrations there can be peroxide burn of the plant material.
Essentially, the products breaks down to water and oxygen gas.
Foliar Application Complete Coverage is Key
With this (and any other contact only product) good plant coverage is very important as this has a direct impact on the effectiveness of the product.
4. Soil Treatment
This is where hydrogen peroxide is showing greater interest and some initial results are the reason for its continued use.
Some areas have strict regulations on products for controlling soil based diseases.
This is for good reason as these can have long residuals and negatively impact non-target environments.
Soil Treatment
Hydrogen peroxide can control a wide range of soil based diseases with low residual so it will not have long term negative impacts on the environment.
It is much easier and cost effective compared to steam sterilization.
While this may be better it is not easy to do and is costly in both labor and equipment.
Soil Treatment Site Preparations
Clear crop residue
Some can be on the surface and/or partially incorporated into the soil, but a lot of plant residue will reduce soil penetration
The soil surface should be quick/rough tilled to allow for greater percolation into the soil profile.
Soil should have some moisture
Post Soil Treatment
Adding back in beneficial microbes
Manufacture says these can be added only 2-hours after the peroxide application.
This is when the foaming you may see on the soil surface during application has gone away.
Soil Treatment Micro Replenish TerraGrow
Following manufacture recommendations, TerraGrow was added to the same area that was treated with the TerraClean product 48-hours prior.
So, be sure to get the entire growing area when performing this treatment.
While it will not be 100% effective the delayed onset and mitigation of most pressure sure makes it more effective than many of the harsher chemical options that exist.
Cover crops are still an important component
For many soil based diseases you want reduce the chance of overwatering and excessive nitrogen fertilizers.
Link to Lecture Slides: drive.google.com/file/d/1AmVG6JHNzen6QqvS4RJkBuiYXq_ELBAQ/view?usp=share_link
*Due to the description character limit the full work cited for "The Many Uses of Hydrogen Peroxide in the Garden" can be viewed at... docs.google.com/document/d/1Ok6TYaGcwmiFpXwbZPg7PvD9ydidvm92/edit?usp=share_link&ouid=104237452697237972847&rtpof=true&sd=true


![How Can You Prevent Hop Latent Viroid (HLVd) From Spreading?
How Can You Prevent Hop Latent Viroid HLVd From Spreading?
Professor DeBacco
Additional Video Sources:
Medicinal Genomics. (2023a, June 8). Understanding and Managing HOP latent viroid in cannabis - Zamir Punja, PhD [Video]. YouTube. https://www.youtube.com/watch?v=xQTRWQuUCzY
Starts with Testing/Screening
Knowing what you have
How Can You Prevent HLVd from Spreading?
Strict and consistent tool sterilization
PPE for workers
Rigorous testing protocol
Repeat testing of Mother room
Plant Eradication
Removing plants that test positive and removing them, followed by continual testing can reduce the presence of HLVd in a growing operation.
Cleaning Tools and Surfaces
This should be done on a regular basis.
However the product you select as your “cleaner” may make the area look clean but many not have any impact on HLVd
Not Effective Methods
Alcohol
Alcohol + flame
Flaming tools
H2O2
Acetic Acid (Vinegar)
Ammonium cleaners (Ex. Lysol)
UV Light
UV-C exposure on leaves and roots for 5min. And viroid was still present
What Does Work… (On Tools and Surfaces)
Virkon S 2%
Household Bleach 10-20% for 30-60sec.
Common lab saying… 10% bleach for 10min.
Mix and use the same day.
Same Can Not Be Applied to Root (Plant) Tissue
Previous products and recommendations are for tools and surfaces and those effective in those situations are not effective at cleaning plant tissue.
UV-C Irradiation
The viroid is very stable in plant tissue.
This makes it very hard to “clean” plant material which is why it should be bagged and physically removed.
Sap is much more Difficult
The “best” method for sap containing viroids may be Nucleases
Link to Lecture Slides: https://drive.google.com/file/d/1bKoKL-iZDk7VL11g46zDLL8cEOAnxuZe/view?usp=drive_link
*Due to the description character limit the full work cited for How Can You Prevent Hop Latent Viroid (HLVd) From Spreading? can be viewed at... https://docs.google.com/document/d/1va7Y3vADzL0X_HS82j2MRWTVNlHf_IFH/edit?usp=drive_link&ouid=104237452697237972847&rtpof=true&sd=true How Can You Prevent Hop Latent Viroid (HLVd) From Spreading?](https://i.ytimg.com/vi/ekSHtVF5eKo/mqdefault.jpg)




![Nitrogen Fertilizers Impact on CBD and THC Concentrations in Cannabis
Nitrogen Fertilizers Impact on CBD and THC Concentrations in Cannabis
Professor DeBacco
Research Article
Dilena, E., Close, D. C., Hunt, I., & Garland, S. M. (2023). Investigating how nitrogen nutrition and pruning impacts on CBD and THC concentration and plant biomass of Cannabis sativa. Scientific Reports, 13(1), 19533.
https://www.nature.com/articles/s41598-023-46369-5
Total Biomass and Total Cannabinoid Concentrations
Total biomass and total cannabinoid concentrations in % DW (on the y-axis) versus measured N concentrations in % DW (on each x-axis).
Treatment group labels are indicated by different colors.
The shape of the points (circle or triangle) indicates whether or not the plant associated with the data point was on the sun edge.
For each regression n = 20.
Inverse Relationship of [N] to [Cannabinoid]
The increase in biomass as N concentration increased from 3 to 6 was approximately 100%
Note the y-axis scale
However, the corresponding decrease in cannabinoid concentrations was approximately 67%
This entails a net decline in yield in terms of grams as N concentration increases
Three Key Points
1. Biomass was strongly and positively related to N
2. Cannabinoid concentrations were strongly and negatively related to N
3. Plants on the sun-edge tended to have higher biomass levels and cannabinoid concentrations
High Nitrogen Levels
Higher nitrogen nutrition raised the concentration of N in both inflorescence and leaf plant matter.
For very high levels of N nutrition (500 mg/mL), inflorescence cannabinoid concentrations decreased significantly (with little change in biomass) and leaf biomass increased significantly.
With higher N supply stem diameter also increased.
The net effect of increasing nitrogen nutrition on the total yield of cannabinoids was negative because the increase in biomass (which was only significant in leaves) was not enough to offset the consequent decrease in cannabinoid concentrations.
Take Home Message…
Cannabinoid concentration, as well as cannabinoid yield per plant were decreased with the increase in N supply.
Target Nitrogen Levels
Very high concentrations of fertilizer are not advisable because of lower cannabinoid concentration and yield, and that the optimal N nutrition is likely to be between 60 and 210 mg/L.
0.008-0.028 ounces per gallon
Prunings Impact
Double stem pruning was applied as an additional treatment to investigate efficacy on biomass increase.
Pruning treatment did not increase cannabinoid concentrations or affect biomass when measured at the final harvest.
Study Also Evaluate Sun Edge Plants
Across all treatments, as an artefact of this trial, sun-edge plants that were more directly exposed to sunlight showed a trend towards more biomass and higher cannabinoid concentrations
According to the statistical model developed for this study.
Self-shading of plants can limit the production of cannabinoids, so it is important to consider plant density and light intensity/spectrum.
*However, these results can only be considered preliminary and does warrants further research.
Link to Lecture Slides: https://drive.google.com/file/d/1lgV_46bDoXmPwXrAQAhfs7b04vcFISMU/view?usp=drive_link
*Due to the description character limit the full work cited for Nitrogen Fertilizers Impact on CBD and THC Concentrations in Cannabis can be viewed at... https://docs.google.com/document/d/1PAyFcsjILnKWvikPChNQmrt3hFAgH5Vh/edit?usp=drive_link&ouid=104237452697237972847&rtpof=true&sd=true Nitrogen Fertilizers Impact on CBD and THC Concentrations in Cannabis](https://i.ytimg.com/vi/gRAM9e2otLg/mqdefault.jpg)


