Uploaded August 2025 | Updated September 2026, 2 weeks ago
Scientific Method Explained
Dr. DeBacco
Scientific Method
Scientific method is a systematic process for investigating phenomena, acquiring new knowledge, or correcting and integrating previous knowledge.
8 Steps to the Scientific Method
Ask a Question
Conduct Background Research
Formulate a Hypothesis
Design and Conduct an Experiment
Analyze Data
Draw Conclusions
Report Results
Refine
1. Ask a Question
Ask a Question: Identify a specific problem or question based on observations or curiosity about the natural world.
The question should be clear and focused.
2. Conduct Background Research
Conduct Background Research: Gather existing information to form a foundation for the investigation and refine the question.
3. Formulate a Hypothesis
Formulate a Hypothesis: Develop a testable, falsifiable statement or prediction based on the research.
4. Design and Conduct an Experiment
Plan a controlled experiment, defining variables (independent, dependent, controls) and procedures.
Perform the experiment, collecting accurate and consistent data.
Independent Variables- The variable you manipulate or control to test its effect. It's the "cause" in a cause-and-effect relationship.
Example: Amount of study time before a test.
Dependent Variables- The variable you measure or test, affected by the independent variable. It's the "effect."
Example: Test score.
Controls- Variables kept constant to prevent them from influencing the results.
Example: Same study material or testing environment for all participants.
5. Analyze Data
Use graphs, statistics, or other tools to interpret data and identify trends.
6. Draw Conclusions
Determine if the hypothesis is supported or refuted, considering the results and limitations.
7. Report Results
Share findings through reports or presentations for others to review or replicate.
8. Refine
Revisit earlier steps based on findings, refining the question, hypothesis, or experiment for further study.
Link to Lecture Slides: drive.google.com/file/d/1f8XrjAN0m2F5oUhrfVhBkyUcMvcLMFXt/view?usp=drive_link
*Due to the description character limit the full work cited for "Scientific Method Explained" can be viewed at... docs.google.com/document/d/1aOWInp0RqLBuxbFw2eAEWNXXUkO50g9Z/edit?usp=drive_link&ouid=104237452697237972847&rtpof=true&sd=true
Scientific Method Explained
Dr. DeBacco
Scientific Method
Scientific method is a systematic process for investigating phenomena, acquiring new knowledge, or correcting and integrating previous knowledge.
8 Steps to the Scientific Method
Ask a Question
Conduct Background Research
Formulate a Hypothesis
Design and Conduct an Experiment
Analyze Data
Draw Conclusions
Report Results
Refine
1. Ask a Question
Ask a Question: Identify a specific problem or question based on observations or curiosity about the natural world.
The question should be clear and focused.
2. Conduct Background Research
Conduct Background Research: Gather existing information to form a foundation for the investigation and refine the question.
3. Formulate a Hypothesis
Formulate a Hypothesis: Develop a testable, falsifiable statement or prediction based on the research.
4. Design and Conduct an Experiment
Plan a controlled experiment, defining variables (independent, dependent, controls) and procedures.
Perform the experiment, collecting accurate and consistent data.
Independent Variables- The variable you manipulate or control to test its effect. It's the "cause" in a cause-and-effect relationship.
Example: Amount of study time before a test.
Dependent Variables- The variable you measure or test, affected by the independent variable. It's the "effect."
Example: Test score.
Controls- Variables kept constant to prevent them from influencing the results.
Example: Same study material or testing environment for all participants.
5. Analyze Data
Use graphs, statistics, or other tools to interpret data and identify trends.
6. Draw Conclusions
Determine if the hypothesis is supported or refuted, considering the results and limitations.
7. Report Results
Share findings through reports or presentations for others to review or replicate.
8. Refine
Revisit earlier steps based on findings, refining the question, hypothesis, or experiment for further study.
Link to Lecture Slides: drive.google.com/file/d/1f8XrjAN0m2F5oUhrfVhBkyUcMvcLMFXt/view?usp=drive_link
*Due to the description character limit the full work cited for "Scientific Method Explained" can be viewed at... docs.google.com/document/d/1aOWInp0RqLBuxbFw2eAEWNXXUkO50g9Z/edit?usp=drive_link&ouid=104237452697237972847&rtpof=true&sd=true



![Nitrogen Fertilizers Impact on CBD and THC Concentrations in Cannabis
Nitrogen Fertilizers Impact on CBD and THC Concentrations in Cannabis
Professor DeBacco
Research Article
Dilena, E., Close, D. C., Hunt, I., & Garland, S. M. (2023). Investigating how nitrogen nutrition and pruning impacts on CBD and THC concentration and plant biomass of Cannabis sativa. Scientific Reports, 13(1), 19533.
https://www.nature.com/articles/s41598-023-46369-5
Total Biomass and Total Cannabinoid Concentrations
Total biomass and total cannabinoid concentrations in % DW (on the y-axis) versus measured N concentrations in % DW (on each x-axis).
Treatment group labels are indicated by different colors.
The shape of the points (circle or triangle) indicates whether or not the plant associated with the data point was on the sun edge.
For each regression n = 20.
Inverse Relationship of [N] to [Cannabinoid]
The increase in biomass as N concentration increased from 3 to 6 was approximately 100%
Note the y-axis scale
However, the corresponding decrease in cannabinoid concentrations was approximately 67%
This entails a net decline in yield in terms of grams as N concentration increases
Three Key Points
1. Biomass was strongly and positively related to N
2. Cannabinoid concentrations were strongly and negatively related to N
3. Plants on the sun-edge tended to have higher biomass levels and cannabinoid concentrations
High Nitrogen Levels
Higher nitrogen nutrition raised the concentration of N in both inflorescence and leaf plant matter.
For very high levels of N nutrition (500 mg/mL), inflorescence cannabinoid concentrations decreased significantly (with little change in biomass) and leaf biomass increased significantly.
With higher N supply stem diameter also increased.
The net effect of increasing nitrogen nutrition on the total yield of cannabinoids was negative because the increase in biomass (which was only significant in leaves) was not enough to offset the consequent decrease in cannabinoid concentrations.
Take Home Message…
Cannabinoid concentration, as well as cannabinoid yield per plant were decreased with the increase in N supply.
Target Nitrogen Levels
Very high concentrations of fertilizer are not advisable because of lower cannabinoid concentration and yield, and that the optimal N nutrition is likely to be between 60 and 210 mg/L.
0.008-0.028 ounces per gallon
Prunings Impact
Double stem pruning was applied as an additional treatment to investigate efficacy on biomass increase.
Pruning treatment did not increase cannabinoid concentrations or affect biomass when measured at the final harvest.
Study Also Evaluate Sun Edge Plants
Across all treatments, as an artefact of this trial, sun-edge plants that were more directly exposed to sunlight showed a trend towards more biomass and higher cannabinoid concentrations
According to the statistical model developed for this study.
Self-shading of plants can limit the production of cannabinoids, so it is important to consider plant density and light intensity/spectrum.
*However, these results can only be considered preliminary and does warrants further research.
Link to Lecture Slides: https://drive.google.com/file/d/1lgV_46bDoXmPwXrAQAhfs7b04vcFISMU/view?usp=drive_link
*Due to the description character limit the full work cited for Nitrogen Fertilizers Impact on CBD and THC Concentrations in Cannabis can be viewed at... https://docs.google.com/document/d/1PAyFcsjILnKWvikPChNQmrt3hFAgH5Vh/edit?usp=drive_link&ouid=104237452697237972847&rtpof=true&sd=true Nitrogen Fertilizers Impact on CBD and THC Concentrations in Cannabis](https://i.ytimg.com/vi/gRAM9e2otLg/mqdefault.jpg)






