Quick Biochemistry Basics | SOLID sequencing | Supported oligonucleotide ligation and detection @quickbiochemistrybasics | Uploaded 3 months ago | Updated 21 hours ago
SOLID stands for Supported Oligonucleotide Ligation and Detection. The method uses short 8 mer sequences for the detection of unknown nucleotide in the given template. out of eight, two nucleotide are very specific while the other are degenerate nucleotide. These sequences are attached with a fluorescence probe. Based on the color of fluorescence we find the unknown sequence.
SOLID stands for Supported Oligonucleotide Ligation and Detection. The method uses short 8 mer sequences for the detection of unknown nucleotide in the given template. out of eight, two nucleotide are very specific while the other are degenerate nucleotide. These sequences are attached with a fluorescence probe. Based on the color of fluorescence we find the unknown sequence.