Quick Biochemistry Basics | Pulsed field gel electrophoresis (PFGE) @quickbiochemistrybasics | Uploaded 2 years ago | Updated 20 hours ago
Pulse field gel electrophoresis is a common technique used for the separation of large DNA fragments and chromosomes. In PFGE the electric field is applied at an angle (across the diagonal) alternatively. As a result of alternate electric field applied at an angle, the large DNA fragments takes a long time to orient themselves in the changing electric field, as compared to short DNA fragments. This allows separation and resolution of DNA fragments.
Pulse field gel electrophoresis is a common technique used for the separation of large DNA fragments and chromosomes. In PFGE the electric field is applied at an angle (across the diagonal) alternatively. As a result of alternate electric field applied at an angle, the large DNA fragments takes a long time to orient themselves in the changing electric field, as compared to short DNA fragments. This allows separation and resolution of DNA fragments.
