Uploaded June 2018 | Updated September 2026, 3 weeks ago
A fluorescence microscope is an optical microscope that uses fluorescence to obeserve the specimen. The specimen is illuminated with light of a specific wavelength (or wavelengths) which is absorbed by the fluorophores / dyes, causing them to emit light of longer wavelengths (i.e., of a different color than the absorbed light). The illumination light is separated from the much weaker emitted fluorescence through the use of a spectral emission filter.
A fluorescence microscope is an optical microscope that uses fluorescence to obeserve the specimen. The specimen is illuminated with light of a specific wavelength (or wavelengths) which is absorbed by the fluorophores / dyes, causing them to emit light of longer wavelengths (i.e., of a different color than the absorbed light). The illumination light is separated from the much weaker emitted fluorescence through the use of a spectral emission filter.
