Quick Biochemistry Basics | Fluorescence Microscopy @quickbiochemistrybasics | Uploaded 6 years ago | Updated 22 hours ago
A fluorescence microscope is an optical microscope that uses fluorescence to obeserve the specimen. The specimen is illuminated with light of a specific wavelength (or wavelengths) which is absorbed by the fluorophores / dyes, causing them to emit light of longer wavelengths (i.e., of a different color than the absorbed light). The illumination light is separated from the much weaker emitted fluorescence through the use of a spectral emission filter.
A fluorescence microscope is an optical microscope that uses fluorescence to obeserve the specimen. The specimen is illuminated with light of a specific wavelength (or wavelengths) which is absorbed by the fluorophores / dyes, causing them to emit light of longer wavelengths (i.e., of a different color than the absorbed light). The illumination light is separated from the much weaker emitted fluorescence through the use of a spectral emission filter.
