Quick Biochemistry Basics | Thin layer chromatography @quickbiochemistrybasics | Uploaded 1 year ago | Updated 1 day ago
Thin layer chromatography is based on adsorption chromatography. In this method, the adsorbent material is applied on the glass plate, plastic surface or an inert solid surface. Once the plate is ready the sample is applied at the end of the plate. The plate is then transferred in a developing chamber that contains mobile phase solvent. The solvent moves up the plate via capillary action. The sample molecules gets separated on the basis of their interaction with the mobile phase and stationary phase.
Thin layer chromatography is based on adsorption chromatography. In this method, the adsorbent material is applied on the glass plate, plastic surface or an inert solid surface. Once the plate is ready the sample is applied at the end of the plate. The plate is then transferred in a developing chamber that contains mobile phase solvent. The solvent moves up the plate via capillary action. The sample molecules gets separated on the basis of their interaction with the mobile phase and stationary phase.
