Quick Biochemistry Basics | Single stranded conformation polymorphism | SSCP @quickbiochemistrybasics | Uploaded 1 year ago | Updated 1 day ago
Single stranded conformation polymorphism is a technique, widely used for the detection of mutations in the DNA. In this method, the samples are amplified by PCR and then heated at 94deg C. The samples are then cooled rapidly. This rapid cooling causes the ssDNA to form base pairs within itself. These structures are then analyzed by gel electrophoresis.

AFLP | Amplified Fragment Length Polymorphism

HPLC | High performance liquid chromatography

$Abbes equation and Numerical aperture Abbe was a scientist who gave the mathematical equation to calculate resolution of microscope. In this equation the denominator n sin alpha, is termed as the numerical aperture. By definition the numerical aperture is the product of refractive index and light gathering ability of objective lens.$

Single stranded conformation polymorphism | SSCP @quickbiochemistrybasics