Reducing sugar by DNS method | 3, 5 Dinitrosalicylic acid (DNSA) method @quickbiochemistrybasics
Reducing sugar by DNS method | 3, 5 Dinitrosalicylic acid (DNSA) method @quickbiochemistrybasics
Uploaded May 2019 | Updated September 2026, 2 weeks ago
3, 5-Dinitrosalicylic acid (DNSA) is used extensively in biochemistry for the estimation of reducing sugars. It detects the presence of free carbonyl group (C=O) of reducing sugars. This involves the oxidation of the aldehyde functional group (in glucose) and the ketone functional group (in fructose).
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Reducing sugar by DNS method | 3, 5 Dinitrosalicylic acid (DNSA) method

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