Quick Biochemistry Basics | Reducing sugar by DNS method | 3, 5 Dinitrosalicylic acid (DNSA) method @quickbiochemistrybasics | Uploaded 5 years ago | Updated 18 hours ago
3, 5-Dinitrosalicylic acid (DNSA) is used extensively in biochemistry for the estimation of reducing sugars. It detects the presence of free carbonyl group (C=O) of reducing sugars. This involves the oxidation of the aldehyde functional group (in glucose) and the ketone functional group (in fructose).
3, 5-Dinitrosalicylic acid (DNSA) is used extensively in biochemistry for the estimation of reducing sugars. It detects the presence of free carbonyl group (C=O) of reducing sugars. This involves the oxidation of the aldehyde functional group (in glucose) and the ketone functional group (in fructose).