Quick Biochemistry Basics | Isoelectric Focusing and 2D gel electrophoresis @quickbiochemistrybasics | Uploaded 2 years ago | Updated 18 hours ago
Iso-Electric focusing is widely used for proteome analysis. Isoelectric focusing is based on the concept of isoelectric pH. The pH at which net charge on protein becomes zero, is called isoelectric pH of protein. Such protein will have equal number of positive and negative charge. As a result net charge become zero. The separation of proteins is carried out on gel with pH gradient. Isoelectric focusing is often followed by electrophoresis in second dimension. This is also known as Two dimensional gel electrophoresis (2D gel electrophoresis)