Uploaded January 2026 | Updated September 2026, 7 hours ago
In this opening address from PAG 33, discover how PacBio HiFi sequencing moves beyond the genome blueprint to reveal true biology through multi-omics.
PacBio Co-Founder Jonas Korlach argues that gene expression counts are insufficient metrics for understanding function and demonstrates why resolving full-length isoforms is critical. He highlights how HiFi sequencing captures the complexity of transcriptomes and mutational processes across the tree of life, contrasting these insights with the limitations of short-read RNA-seq.
*What you’ll learn*
* Evaluate why gene expression counts are insufficient for understanding biological function.
* Examine evidence showing how short-read RNA-seq fails to capture alternative splicing complexity.
* Explore recent discoveries in zebrafish and the Darwin Tree of Life project enabled by HiFi data.
*Featured speakers*
* Jonas Korlach, Co-Founder & Scientific Advisor (PacBio)
00:00 Opening
01:36 Welcome to PAG 33
02:32 From blueprint to true biology
04:06 Why isoforms matter
05:50 The short-read problem
06:20 Iso-Seq discoveries
08:17 Scaling to populations
09:05 Darwin Tree of Life study
10:18 Workshop preview
How is isoform resolution changing your understanding of your organism? *Comment below.*
*Beyond the Bench* a series celebrating the people behind scientific progress:
pacb.com/blog/beyond-the-bench-a-series-celebrating-the-people-behind-scientific-progress
*Subscribe for more PacBio genomics content:*
youtube.com/@PacificBiosciences?sub_confirmation=1
Learn more about PacBio at pacb.com
Legal & Trademarks: pacb.com/legal-and-trademarks
#PAG33 #HiFiSequencing #Genomics
In this opening address from PAG 33, discover how PacBio HiFi sequencing moves beyond the genome blueprint to reveal true biology through multi-omics.
PacBio Co-Founder Jonas Korlach argues that gene expression counts are insufficient metrics for understanding function and demonstrates why resolving full-length isoforms is critical. He highlights how HiFi sequencing captures the complexity of transcriptomes and mutational processes across the tree of life, contrasting these insights with the limitations of short-read RNA-seq.
*What you’ll learn*
* Evaluate why gene expression counts are insufficient for understanding biological function.
* Examine evidence showing how short-read RNA-seq fails to capture alternative splicing complexity.
* Explore recent discoveries in zebrafish and the Darwin Tree of Life project enabled by HiFi data.
*Featured speakers*
* Jonas Korlach, Co-Founder & Scientific Advisor (PacBio)
00:00 Opening
01:36 Welcome to PAG 33
02:32 From blueprint to true biology
04:06 Why isoforms matter
05:50 The short-read problem
06:20 Iso-Seq discoveries
08:17 Scaling to populations
09:05 Darwin Tree of Life study
10:18 Workshop preview
How is isoform resolution changing your understanding of your organism? *Comment below.*
*Beyond the Bench* a series celebrating the people behind scientific progress:
pacb.com/blog/beyond-the-bench-a-series-celebrating-the-people-behind-scientific-progress
*Subscribe for more PacBio genomics content:*
youtube.com/@PacificBiosciences?sub_confirmation=1
Learn more about PacBio at pacb.com
Legal & Trademarks: pacb.com/legal-and-trademarks
#PAG33 #HiFiSequencing #Genomics
