ISSCR 2021 Day 5 | The Stem Cell Podcast @STEMCELLTechnologies

Uploaded June 2021 | Updated September 2026, 10 hours ago

Join Drs. Daylon James, Arun Sharma, and Nicolas Rivron as they discuss the latest research presented at the 2021 ISSCR annual meeting taking place from June 21- 26.

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ISSCR 2021 Day 5 | The Stem Cell Podcast

$How to Isolate Untouched Human NK Cells and Tips for High Recovery In this technical protocol video, you will learn how to isolate untouched human natural killer (NK) cells from a mononuclear cell suspension using the EasySep™ Human NK Cell Isolation Kit (Catalog # 17955) and the The Big Easy EasySep™ Magnet (Catalog # 18001). The video includes optional steps to obtain higher NK cell recovery. This video is for demonstration purposes only; please consult the Product Information Sheet for further details on the protocol. EasySep™ Human NK Cell Isolation Kit (Catalog # 17955) https://www.stemcell.com/technical-resources/educational-materials/videos-and-webinars/how-to-isolate-human-regulatory-t-cells-tregs-using-the-easy-50-easysep-magnet.html?utm_source=youtube&utm_medium=link&utm_campaign=csi_immunology&utm_content=video_nkeasysephttps://www.stemcell.com/easysep-human-nk-cell-isolation-kit.html?utm_source=youtube&utm_medium=link&utm_campaign=cs_immunology&utm_content=nkvideo The Big Easy EasySep™ Magnet (Catalog # 18001) https://www.stemcell.com/products/the-big-easy-easysep-magnet.html Product Information Sheet: https://cdn.stemcell.com/media/files/pis/10000005299-PIS_01.pdf #EasySep #NaturalKillerCell #NKcell #NKcellisolation #CellSeparation #CellIsolation 0:00 - Intro 0:57 - Part I: Isolation of natural killer (NK) cells 2:39 - Addition of the EasySep™ Human NK Cell Isolation Cocktail (Catalog # 17955C) 4:12 - Addition of the EasySep™ Dextran RapidSpheres™ (Component # 50103) 6:13 - Top up of sample with EasySep™ Buffer (Catalog # 20144) and magnetic separation 7:04 - Collection of untouched NK cells 8:06 - Part II: Optional steps to achieve higher recovery 9:19 - Resuspension of EasySep™ Dextran RapidSpheres™ (Component # 50103) bound cell fraction and magnetic separation 11:14 - Collection of additional untouched NK cells 12:23 - Additional suggestion for higher recovery Below are more resources to learn how to prepare your samples for cell isolation: How to use SepMate™ to isolate peripheral blood mononuclear cells (PBMCs) from whole blood: https://www.youtube.com/watch?v=FM-7BwR0T9c How to recover cells from a leukocyte reduction system (LRS) cone: https://www.youtube.com/watch?v=k6vPSnJvudc How to process a leukopak for downstream cell isolation with EasySep™: https://www.stemcell.com/technical-resources/educational-materials/protocols/leukopak-processing-protocol.html Insights and techniques for efficient leukopak sample processing: https://www.stemcell.com/technical-resources/leukopak-processing-tips-and-tricks-for-streamlined-cell-isolation.html For a full list of products as well as educational resources, please visit our website: https://www.stemcell.com Follow us on Twitter: https://bit.ly/3jW20Gv Like us on Facebook: https://bit.ly/32ern07 Follow us on LinkedIn: https://bit.ly/32uKovJ Subscribe to our YouTube channel: https://bit.ly/32f28ei$

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