Uploaded December 2021 | Updated September 2026, 3 days ago

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In this technical protocol video, you will learn how to isolate untouched human natural killer (NK) cells from a mononuclear cell suspension using the EasySep™ Human NK Cell Isolation Kit (Catalog # 17955) and the "The Big Easy" EasySep™ Magnet (Catalog # 18001). The video includes optional steps to obtain higher NK cell recovery. This video is for demonstration purposes only; please consult the Product Information Sheet for further details on the protocol.
EasySep™ Human NK Cell Isolation Kit (Catalog # 17955) stemcell.com/technical-resources/educational-materials/videos-and-webinars/how-to-isolate-human-regulatory-t-cells-tregs-using-the-easy-50-easysep-magnet.html?utm_source=youtube&utm_medium=link&utm_campaign=csi_immunology&utm_content=video_nkeasysephttps://www.stemcell.com/easysep-human-nk-cell-isolation-kit.html?utm_source=youtube&utm_medium=link&utm_campaign=cs_immunology&utm_content=nkvideo
"The Big Easy" EasySep™ Magnet (Catalog # 18001)
stemcell.com/products/the-big-easy-easysep-magnet.html
Product Information Sheet: cdn.stemcell.com/media/files/pis/10000005299-PIS_01.pdf
#EasySep #NaturalKillerCell #NKcell #NKcellisolation #CellSeparation #CellIsolation

0:00 - Intro
0:57 - Part I: Isolation of natural killer (NK) cells
2:39 - Addition of the EasySep™ Human NK Cell Isolation Cocktail (Catalog # 17955C)
4:12 - Addition of the EasySep™ Dextran RapidSpheres™ (Component # 50103)
6:13 - Top up of sample with EasySep™ Buffer (Catalog # 20144) and magnetic separation
7:04 - Collection of untouched NK cells
8:06 - Part II: Optional steps to achieve higher recovery
9:19 - Resuspension of EasySep™ Dextran RapidSpheres™ (Component # 50103) bound cell fraction and magnetic separation
11:14 - Collection of additional untouched NK cells
12:23 - Additional suggestion for higher recovery

Below are more resources to learn how to prepare your samples for cell isolation:

How to use SepMate™ to isolate peripheral blood mononuclear cells (PBMCs) from whole blood: youtube.com/watch?v=FM-7BwR0T9c
How to recover cells from a leukocyte reduction system (LRS) cone: youtube.com/watch?v=k6vPSnJvudc
How to process a leukopak for downstream cell isolation with EasySep™: stemcell.com/technical-resources/educational-materials/protocols/leukopak-processing-protocol.html
Insights and techniques for efficient leukopak sample processing: stemcell.com/technical-resources/leukopak-processing-tips-and-tricks-for-streamlined-cell-isolation.html

For a full list of products as well as educational resources, please visit our website: stemcell.com

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