Uploaded August 2025 | Updated September 2026, 1 week ago
Hund’s Rule
Dr. DeBacco
Hund’s Rule
“Empty seat on the bus rule”
Fill one space in each orbital within a sublevel before filling the second seat in an orbital
When electrons occupy orbitals of equal energy (like the three 2p orbitals), they:
Fill each orbital singly first (↑ ↑ ↑)
Then begin to pair up (↑↓ ↑ ↑)
This minimizes electron repulsion and increases stability.
Hund’s Rule
Hund’s Rule Examples
Link to Lecture Slides: drive.google.com/file/d/1rTqdkFHrGyfAhzXEmnrFOvI7SyJeK9bt/view?usp=drive_link
*Due to the description character limit the full work cited for "Hund's Rule" can be viewed at... docs.google.com/document/d/1lRQN1V2sSmKiKFQRzdvE8RshxbeXC651/edit?usp=drive_link&ouid=104237452697237972847&rtpof=true&sd=true
Hund’s Rule
Dr. DeBacco
Hund’s Rule
“Empty seat on the bus rule”
Fill one space in each orbital within a sublevel before filling the second seat in an orbital
When electrons occupy orbitals of equal energy (like the three 2p orbitals), they:
Fill each orbital singly first (↑ ↑ ↑)
Then begin to pair up (↑↓ ↑ ↑)
This minimizes electron repulsion and increases stability.
Hund’s Rule
Hund’s Rule Examples
Link to Lecture Slides: drive.google.com/file/d/1rTqdkFHrGyfAhzXEmnrFOvI7SyJeK9bt/view?usp=drive_link
*Due to the description character limit the full work cited for "Hund's Rule" can be viewed at... docs.google.com/document/d/1lRQN1V2sSmKiKFQRzdvE8RshxbeXC651/edit?usp=drive_link&ouid=104237452697237972847&rtpof=true&sd=true









![How Can You Prevent Hop Latent Viroid (HLVd) From Spreading?
How Can You Prevent Hop Latent Viroid HLVd From Spreading?
Professor DeBacco
Additional Video Sources:
Medicinal Genomics. (2023a, June 8). Understanding and Managing HOP latent viroid in cannabis - Zamir Punja, PhD [Video]. YouTube. https://www.youtube.com/watch?v=xQTRWQuUCzY
Starts with Testing/Screening
Knowing what you have
How Can You Prevent HLVd from Spreading?
Strict and consistent tool sterilization
PPE for workers
Rigorous testing protocol
Repeat testing of Mother room
Plant Eradication
Removing plants that test positive and removing them, followed by continual testing can reduce the presence of HLVd in a growing operation.
Cleaning Tools and Surfaces
This should be done on a regular basis.
However the product you select as your “cleaner” may make the area look clean but many not have any impact on HLVd
Not Effective Methods
Alcohol
Alcohol + flame
Flaming tools
H2O2
Acetic Acid (Vinegar)
Ammonium cleaners (Ex. Lysol)
UV Light
UV-C exposure on leaves and roots for 5min. And viroid was still present
What Does Work… (On Tools and Surfaces)
Virkon S 2%
Household Bleach 10-20% for 30-60sec.
Common lab saying… 10% bleach for 10min.
Mix and use the same day.
Same Can Not Be Applied to Root (Plant) Tissue
Previous products and recommendations are for tools and surfaces and those effective in those situations are not effective at cleaning plant tissue.
UV-C Irradiation
The viroid is very stable in plant tissue.
This makes it very hard to “clean” plant material which is why it should be bagged and physically removed.
Sap is much more Difficult
The “best” method for sap containing viroids may be Nucleases
Link to Lecture Slides: https://drive.google.com/file/d/1bKoKL-iZDk7VL11g46zDLL8cEOAnxuZe/view?usp=drive_link
*Due to the description character limit the full work cited for How Can You Prevent Hop Latent Viroid (HLVd) From Spreading? can be viewed at... https://docs.google.com/document/d/1va7Y3vADzL0X_HS82j2MRWTVNlHf_IFH/edit?usp=drive_link&ouid=104237452697237972847&rtpof=true&sd=true How Can You Prevent Hop Latent Viroid (HLVd) From Spreading?](https://i.ytimg.com/vi/ekSHtVF5eKo/mqdefault.jpg)
