Uploaded September 2020 | Updated September 2026, 1 hour ago

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In this protocol video, you will learn how to expand human bronchial epithelial cells (HBECs) to initiate an air-liquid interface (ALI) culture using PneumaCult™ culture media.

00:00 Introduction
00:35 Phase 1: Expansion Culture in Flasks
01:37 Plating
02:45 Morphological Assessment
05:36 Phase 2: Submerged Expansion in Inserts


Watch the other videos in this series:

How to Model the Human Airway at the Air-Liquid Interface: Introduction - youtu.be/u2OLfPT8qIw
How to Model the Human Airway at the Air-Liquid Interface: Differentiation of HBECs - youtu.be/MbXwvBWQv0A


Explore additional resources for your research:

How to Count Cells with a Hemocytometer
bit.ly/3iiFV4N

Try PneumaCult™ in your Lab
bit.ly/32mneYN

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