STEMCELL Technologies
How to Model the Human Airway at the Air-Liquid interface: Introduction
updated
Timecode:
0:00 Intro
0:26 Preparing required reagents and materials
1:42 Clump passaging a 3D hPSC culture using GCDR and filter-based trituration
5:09 Seeding hPSC clumps into a 6-well plate
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0:00 Intro
0:32 Performing a fed-batch feed
0:58 Performing a 50% medium exchange
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#ThawSTAR #thawfrozencells #cellthawing
stemcell.com/easy-250-easysep-magnet.html
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#EasySep #CellSeparation #Easy250
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stemcell.com/easy-250-easysep-magnet.html
#EasySep #CellSeparation #CellIsolation #Largevolume #Easy250
0:00 Intro
01:31 Adjusting the base platform height to fit your T-75 cm2 flask
03:06 Cell Isolation Cocktail, Magnetic Particles (RapidSpheres™) and Buffer addition
08:10 Collecting the supernatant
10:19 Re-suspension of positively selected cells
Below are more resources to learn how to prepare your samples for cell isolation:
How to process a leukopak for downstream cell isolation with EasySep™:
stemcell.com/technical-resources/educational-materials/protocols/leukopak-processing-protocol.html
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Official Website: immunologypodcast.com
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#ImmunologyPodcast
This podcast is brought to you by STEMCELL Technologies!
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#StemCellPodcast
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00:00 Intro
00:58 Transferring the Contents
05:03 ‘Wash & Go’ Protocol - washing protocol without RBC lysis and platelet removal
09:22 ‘Lyse & Go’ Protocol - RBC Lysis and Platelet Removal
13:15 Resources
Interested in learning more?
Watch our on-demand leukopak processing webinar at bit.ly/3Ar7rqU
To learn more about cell sourcing and human primary cells, visit bit.ly/3jzW71A
Explore frequently asked questions on primary cells at bit.ly/31OhW7H
Request information about introductory offers to try human primary cell products at bit.ly/32x4AAd
For more information on cell separation using EasySep™, visit bit.ly/31NYfwq
For a full list of products, as well as educational resources, visit our website: stemcell.com
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#leukopakprocessing #pbmcisolation #STEMCELLTechnologies
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0:00 Intro and Roundup
29:57 Guest Interview
#Hematopoiesis #IRBBarcelona #AlejoRodriguezFraticelli
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Official Website: immunologypodcast.com
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0:00 Intro and Roundup
36:08 Guest Interview
#UterineImmunity #Cambridge #FrancescoColucci
This podcast is brought to you by STEMCELL Technologies!
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0:00 Intro and Roundup
33:01 Guest Interview
#CardiacDisease #NazishSayed #StandfordResearch
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Eric Toombs is a Senior Research Technologist at STEMCELL Technologies. This poster was first presented at the American Association of Immunologists’ (AAI) 2021 annual meeting.
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#easy250 #cellisolation #stemcelltechnologies
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0:00 Intro and Roundup
30:13 Guest Interview
#TrainedImmunity #Epigenetics #MihaiNetea
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0:00 Intro and Roundup
30:08 Guest Interview
#AllenInstitute #RuGunawardane #KaytlynGerbin
This podcast is brought to you by STEMCELL Technologies!
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0:00 Intro and Roundup
33:35 Guest Interview
#BCells #NasalVaccines #Antibodies
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0:00 Intro and Roundup
38:11 Guest Interview
#Biomanufacturing #IlyasSingeç #NIH
This podcast is brought to you by STEMCELL Technologies!
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0:00 Intro and Roundup
36:12 Guest Interview
#Helminths #Immunomodulation #DiversityEquityInclusion
This podcast is brought to you by STEMCELL Technologies!
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0:00 Preparation
1:08 Transfer Spleen Fragments
2:15 Discard Supernatant
#EasySep Magnet #EasySep #STEMCELL Technologies
EasySep™ Human NK Cell Isolation Kit (Catalog # 17955) stemcell.com/technical-resources/educational-materials/videos-and-webinars/how-to-isolate-human-regulatory-t-cells-tregs-using-the-easy-50-easysep-magnet.html?utm_source=youtube&utm_medium=link&utm_campaign=csi_immunology&utm_content=video_nkeasysepstemcell.com/easysep-human-nk-cell-isolation-kit.html?utm_source=youtube&utm_medium=link&utm_campaign=cs_immunology&utm_content=nkvideo
"The Big Easy" EasySep™ Magnet (Catalog # 18001)
stemcell.com/products/the-big-easy-easysep-magnet.html
Product Information Sheet: cdn.stemcell.com/media/files/pis/10000005299-PIS_01.pdf
#EasySep #NaturalKillerCell #NKcell #NKcellisolation #CellSeparation #CellIsolation
0:00 - Intro
0:57 - Part I: Isolation of natural killer (NK) cells
2:39 - Addition of the EasySep™ Human NK Cell Isolation Cocktail (Catalog # 17955C)
4:12 - Addition of the EasySep™ Dextran RapidSpheres™ (Component # 50103)
6:13 - Top up of sample with EasySep™ Buffer (Catalog # 20144) and magnetic separation
7:04 - Collection of untouched NK cells
8:06 - Part II: Optional steps to achieve higher recovery
9:19 - Resuspension of EasySep™ Dextran RapidSpheres™ (Component # 50103) bound cell fraction and magnetic separation
11:14 - Collection of additional untouched NK cells
12:23 - Additional suggestion for higher recovery
Below are more resources to learn how to prepare your samples for cell isolation:
How to use SepMate™ to isolate peripheral blood mononuclear cells (PBMCs) from whole blood: youtube.com/watch?v=FM-7BwR0T9c
How to recover cells from a leukocyte reduction system (LRS) cone: youtube.com/watch?v=k6vPSnJvudc
How to process a leukopak for downstream cell isolation with EasySep™: stemcell.com/technical-resources/educational-materials/protocols/leukopak-processing-protocol.html
Insights and techniques for efficient leukopak sample processing: stemcell.com/technical-resources/leukopak-processing-tips-and-tricks-for-streamlined-cell-isolation.html
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0:00 Preparation
1:32 Transfer Lung Tissue
2:01 Discard Supernatant
#EasySep #EasySep Magnet #STEMCELL Technologies
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0:00 Intro and Roundup
36:43 Guest Interview
#Mechanotransduction #SaraWickström #UniversityofHelsinki
This podcast is brought to you by STEMCELL Technologies!
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Official Website: immunologypodcast.com
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0:00 Intro and Roundup
30:01 Guest Interview
#BCells #Microscopy #Osteomorphs
This podcast is brought to you by STEMCELL Technologies!
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0:00 Preparation
1:14 Transfer Brain Tissue
1:47 Discard supernatant
#EasySep Magnet #EasySep #STEMCELL Technologies
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0:00 Intro and Roundup
32:24 Guest Interview
#Cardiomyocytes #ChristineMummery #LeidenUniversity
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0:00 Intro and Roundup
42:06 Guest Interview
#InnateImmunity #Immunometabolism #JennyTing
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0:00 Intro and Roundup
30:37 Guest Interview
#NASA #KateRubins #InternationalSpaceStation
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#hPSCs #embryonicstemcells #cellbanking
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0:00 Intro and Roundup
35:26 Guest Interview
#TCells #Tregs #DarioVignali
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0:00 Intro and Roundup
31:11 Guest Interview
#RegenerativeMedicine #CIRM #MariaMillan
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0:00 Intro and Roundup
35:42 Guest Interview
#Senescence #Aging #Diabetes
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0:00 Intro and Roundup
29:25 Guest Interview
#StemCells #ScienceCommunication #PaulKnoepfler
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0:00 Intro and Roundup
33:53 Guest Interview
#NeuroImmunology #Behavior #SexDifferences
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0:00 Intro and Roundup
26:50 Guest Interview
#HematopoieticStemCells #JacksonLaboratory #JenniferTrowbridge
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0:00 Intro and Roundup
31:05 Guest Interview
#BCells #NasalVaccines #Antibodies
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0:00 Intro and Roundup
31:33 Dr. Leili Rohani
50:45 Dr. Bria Macklin
1:02:12 Dr. Matthew Sinton
#Trainees #LeiliRohani #BriaMacklin #MatthewSinton
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0:00 Intro and Roundup
33:01 Guest Interview
#Tregs #Immunotherapy #SonomaBiotherapeutics
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0:00 Intro and Roundup
28:31 Guest Interview
#BreastCancer #Leukemia #ConnieEaves
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0:00 Intro and Roundup
29:25 Guest Interview
#Immunotherapy #TCells #Neoantigens
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0:00 Intro and Roundup
27:32 Guest Interview
#Regeneration #Zebrafish #KenPoss
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0:00 Intro and Roundup
31:00 Guest Interview
#MonoclonalAntibodies #InfectiousDisease
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0:00 Intro and Roundup
29:42 Guest Interview
#RegenerativeMedicine #TissueEngineering #TheStemCellPodcast
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In this wallchart you'll find:
Key takeaways from studies applying organoids to human health and disease
Examples of organoids as model systems to study infectious disease, cancer, congenital disorders, and tissue regeneration
Potential therapeutic applications of organoids
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0:00 Intro and Roundup
31:35 Guest Interview
#Lymphocytes #TCellActivation #CD4
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0:00 Intro and Roundup
30:12 Guest Interview
#RegenerativeMedicine #SciencePolicy #BernieSiegel
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