Uploaded May 2020 | Updated September 2026, 1 hour ago

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In this series of three technical videos, we will guide you through the protocol for transitioning human pluripotent stem cells (hPSCs) cultured in feeder-free media, such as mTeSR™1, into mTeSR™ Plus.

This video will cover the generation of cell aggregates and describe a passaging protocol using ReLeSR™ or gentle cell dissociation reagent (GCDR).

0:00 - Intro
1:23 - ReLeSR™ Passaging
3:14 - GCDR Passaging
5:56 - Plate Setup for mTeSR™ Plus Transition

Key topics covered in this video:
* Assessing quality of hPSC cultures through morphology using phase-contrast microscopy to determine when to passage
* Passaging cell aggregates using ReLeSR™ or GCDR
* Optimizing aggregate size for feeder-free PSC culture
* Plating aggregates into maintenance medium

Watch the other two videos in the series below:
Video 1: Plate Coating for hPSC Cultures - youtu.be/7kjJ5Iprfoo
Video 3: Maintenance and Morphological Assessment - youtu.be/3gtec8e1qSA

Learn more about mTeSR™ Plus hPSC maintenance media: stemcell.com/why-mtesrplus
View a written protocol on how to transition hPSCs from feeder-free media to mTeSR™ Plus: stemcell.com/transitioning-pscs-to-mtesr-plus.html
Learn more about passaging without scraping with ReLeSR™: stemcell.com/relesr.html

#hPSCculture #HumanPluripotentStemCells #mTeSRPlus

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