Uploaded July 2025 | Updated September 2026, 2 weeks ago
Green Fluorescent Protein (GFP) is a well-studied protein that exhibits fluorescence when exposed to ultraviolet or blue light, making it a powerful tool in molecular biology and biotechnology. Learn how to explore GFP using tools from the RCSB PDB with this tutorial.
Additional resources for this tutorial are available at pdb101.rcsb.org/learn/other-resources/exploring-green-fluorescent-protein-tutorial
Video Chapters:
0:00 Introduction - Using Mol* to Explore Protein Sequence and Structure using Green Fluorescent Protein
0:40 Search for Green Fluorescent Protein entries in the RCSB PDB
0:54 Begin with the Structure Summary Page of entry 1EMA
1:11 Open the Mol* Viewer and highlight an amino acid in the sequence
1:43 Switch to a ball-and-stick view
2:30 Zoom in to view all atoms and bonds
3:09 Isolate the chromophore
4:13 Investigate the hydrogen bonds stabilizing the structure
Green Fluorescent Protein (GFP) is a well-studied protein that exhibits fluorescence when exposed to ultraviolet or blue light, making it a powerful tool in molecular biology and biotechnology. Learn how to explore GFP using tools from the RCSB PDB with this tutorial.
Additional resources for this tutorial are available at pdb101.rcsb.org/learn/other-resources/exploring-green-fluorescent-protein-tutorial
Video Chapters:
0:00 Introduction - Using Mol* to Explore Protein Sequence and Structure using Green Fluorescent Protein
0:40 Search for Green Fluorescent Protein entries in the RCSB PDB
0:54 Begin with the Structure Summary Page of entry 1EMA
1:11 Open the Mol* Viewer and highlight an amino acid in the sequence
1:43 Switch to a ball-and-stick view
2:30 Zoom in to view all atoms and bonds
3:09 Isolate the chromophore
4:13 Investigate the hydrogen bonds stabilizing the structure
