Uploaded November 2025 | Updated September 2026, 3 hours ago

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*How does PacBio HiFi sequencing detect epigenetic modifications like 5mC and 6mA from a single experiment?* This animated explainer shows how the technology works at the single-molecule level.

PacBio long-read sequencing measures the speed of the DNA polymerase as it incorporates bases. This kinetic data can be used to natively detect modified bases like 5-methylcytosine (5mC) and N6-methyladenine (6mA) without special sample preparation, enabling multiomic studies from one assay.

*What you’ll learn*
* How polymerase kinetics are used to identify modified nucleotides.
* The role of promoter hypermethylation in silencing tumor suppressor genes.
* How the Fiber-seq assay reveals haplotype-specific chromatin accessibility.

*Resources*
* Learn more about epigenomics with PacBio: pacb.com/products-and-services/applications/epigenetics

What epigenetic questions could you answer with native methylation detection? *Comment below.*

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#Epigenetics #LongReadSequencing #DNAMethylation