Uploaded April 2026 | Updated September 2026, 14 hours ago
Lower cost. Same workflow. SPRQ-Nx is PacBio's multi-use SMRT Cell chemistry for HiFi long-read sequencing on The Revio System. Reuse a single cell across multiple acquisitions to get more sequencing out of every cell, with automated washing that happens on-instrument.
If you're already running SPRQ, adding SPRQ-Nx is an easy upgrade. Same SMRTbell library prep. Same polymerase kit. Same HiFi data you trust. The only thing that changes is a single dropdown in Run Design, and what you pay per genome.
*Common questions*
- Do I need new hardware for SPRQ-Nx? No. It runs on every existing Revio System, no instrument upgrade or recalibration required.
- Is the library prep different? No. Same SMRTbell library prep, same polymerase kit, same HiFi data. The only change is a single dropdown in Run Design.
- How much does it save? About 30% lower reagent cost per genome versus standard SPRQ¹. For labs running 2+ sequencing runs per week, that adds up fast over a year.
¹ Reagent cost only. Excludes instrument amortization and other reagents. Cost may vary by region. USD pricing. Study design, sample type, and level of multiplexing may affect the number of SMRT Cells required.
*Presenter*
- Samantha Kalla, Field Application Scientist (PacBio)
*Chapters*
00:00 What's new (and what isn't) with SPRQ-Nx
00:18 Compatibility and consumables (SPRQ vs. SPRQ-Nx)
00:58 Prepping the sequencing plates
01:22 Run Design in SMRT Link
02:32 Loading the Revio work deck
03:15 Automated washing between acquisitions
03:37 Pre-loading for continuous runs
*Resources*
- pacb.com/revio
- pacb.com/revio/#sprq-nx
- pacb.com/technology/hifi-sequencing
*Subscribe for more PacBio genomics content:*
youtube.com/@PacificBiosciences?sub_confirmation=1
Learn more about PacBio at pacb.com
Legal & Trademarks: pacb.com/legal-and-trademarks
#SPRQNx #RevioSystem #HiFiLongReadSequencing
Lower cost. Same workflow. SPRQ-Nx is PacBio's multi-use SMRT Cell chemistry for HiFi long-read sequencing on The Revio System. Reuse a single cell across multiple acquisitions to get more sequencing out of every cell, with automated washing that happens on-instrument.
If you're already running SPRQ, adding SPRQ-Nx is an easy upgrade. Same SMRTbell library prep. Same polymerase kit. Same HiFi data you trust. The only thing that changes is a single dropdown in Run Design, and what you pay per genome.
*Common questions*
- Do I need new hardware for SPRQ-Nx? No. It runs on every existing Revio System, no instrument upgrade or recalibration required.
- Is the library prep different? No. Same SMRTbell library prep, same polymerase kit, same HiFi data. The only change is a single dropdown in Run Design.
- How much does it save? About 30% lower reagent cost per genome versus standard SPRQ¹. For labs running 2+ sequencing runs per week, that adds up fast over a year.
¹ Reagent cost only. Excludes instrument amortization and other reagents. Cost may vary by region. USD pricing. Study design, sample type, and level of multiplexing may affect the number of SMRT Cells required.
*Presenter*
- Samantha Kalla, Field Application Scientist (PacBio)
*Chapters*
00:00 What's new (and what isn't) with SPRQ-Nx
00:18 Compatibility and consumables (SPRQ vs. SPRQ-Nx)
00:58 Prepping the sequencing plates
01:22 Run Design in SMRT Link
02:32 Loading the Revio work deck
03:15 Automated washing between acquisitions
03:37 Pre-loading for continuous runs
*Resources*
- pacb.com/revio
- pacb.com/revio/#sprq-nx
- pacb.com/technology/hifi-sequencing
*Subscribe for more PacBio genomics content:*
youtube.com/@PacificBiosciences?sub_confirmation=1
Learn more about PacBio at pacb.com
Legal & Trademarks: pacb.com/legal-and-trademarks
#SPRQNx #RevioSystem #HiFiLongReadSequencing
