Uploaded May 2025 | Updated September 2026, 5 hours ago
Discover PacBio's native 5mC and 6mA methylation detection using SMRT sequencing. Learn how HiFi reads encode this crucial epigenetic data in BAM file MM and ML tags.
PacBio SMRT sequencing offers multi-omic insights, including direct detection of base modifications like 5mC and human 6mA from a single run, eliminating the need for bisulfite treatment. This is achieved by analyzing raw sequencing kinetics (pulse-width and interpulse duration) altered by modified bases.
Learn how PacBio's on-instrument algorithms automatically call 5mC and 6mA, storing these calls and their probabilities directly in the MM and ML tags of HiFi read BAM files. Understand the encoding schemes for accurate downstream bioinformatics analysis.
For downstream analysis:
* 5mC: pb-CpG-tools, MethBat (from PacBio GitHub)
* Human 6mA (treated libraries): Third-party Fiber-seq tools
* Microbial 4mC & 6mA: SMRT Link workflow or HiFi-ASM with methylation-motif calling
Subscribe for more bioinformatics insights and comment below with your questions!
youtube.com/@PacificBiosciences?sub_confirmation=1
Discover PacBio's native 5mC and 6mA methylation detection using SMRT sequencing. Learn how HiFi reads encode this crucial epigenetic data in BAM file MM and ML tags.
PacBio SMRT sequencing offers multi-omic insights, including direct detection of base modifications like 5mC and human 6mA from a single run, eliminating the need for bisulfite treatment. This is achieved by analyzing raw sequencing kinetics (pulse-width and interpulse duration) altered by modified bases.
Learn how PacBio's on-instrument algorithms automatically call 5mC and 6mA, storing these calls and their probabilities directly in the MM and ML tags of HiFi read BAM files. Understand the encoding schemes for accurate downstream bioinformatics analysis.
For downstream analysis:
* 5mC: pb-CpG-tools, MethBat (from PacBio GitHub)
* Human 6mA (treated libraries): Third-party Fiber-seq tools
* Microbial 4mC & 6mA: SMRT Link workflow or HiFi-ASM with methylation-motif calling
Subscribe for more bioinformatics insights and comment below with your questions!
youtube.com/@PacificBiosciences?sub_confirmation=1
