Uploaded April 2021 | Updated September 2026, 1 week ago
Cellular reprogramming is of great interest for many reasons; studying human diseases, understanding cellular plasticity and epigenetic cell state and also for regenerative medicine such as cellular replacement or in vivo reprogramming.
Expression of Yamanaka factors was shown previously to reprogram fibroblasts cells. Expression was achieved by having exogenous expression of these genes. What if endogenous activation of these genes could be used? Well, it has been done and cellular reprogramming has been achieved by synthetic biology approaches, notably as we will discuss in this video by engineered CRISPR tools, such as CRISPR activation (CRISPRa) and CRISPR interference (CRISPRi). These tools function as synthetic transcription factors and can be used to activate or repress gene expression, respectively.
TIMESTAMPS;
Intro - 00:00
CRISPR tools - 01:30
Cellular reprogramming - 04:45
Using tools for reprogramming - 07:10
REFERENCES;
Weltner, J., Balboa, D., Katayama, S. et al. Human pluripotent reprogramming with CRISPR activators. Nat Commun 9, 2643 (2018). doi.org/10.1038/s41467-018-05067-x
CRISPR-Based Chromatin Remodeling... doi.org/10.1016/j.stem.2017.12.001
Review article where figures are from doi.org/10.1152/physrev.00034.2019
Engineering cell fate - doi.org/10.1016/j.coisb.2020.09.002
Check out @LongevityScienceNews latest video - youtube.com/watch?v=I5IypXnjHok
Icons in intro; "freepik.com/free-photos-vectors/background"Background vector created by freepik - freepik.com
Cellular reprogramming is of great interest for many reasons; studying human diseases, understanding cellular plasticity and epigenetic cell state and also for regenerative medicine such as cellular replacement or in vivo reprogramming.
Expression of Yamanaka factors was shown previously to reprogram fibroblasts cells. Expression was achieved by having exogenous expression of these genes. What if endogenous activation of these genes could be used? Well, it has been done and cellular reprogramming has been achieved by synthetic biology approaches, notably as we will discuss in this video by engineered CRISPR tools, such as CRISPR activation (CRISPRa) and CRISPR interference (CRISPRi). These tools function as synthetic transcription factors and can be used to activate or repress gene expression, respectively.
TIMESTAMPS;
Intro - 00:00
CRISPR tools - 01:30
Cellular reprogramming - 04:45
Using tools for reprogramming - 07:10
REFERENCES;
Weltner, J., Balboa, D., Katayama, S. et al. Human pluripotent reprogramming with CRISPR activators. Nat Commun 9, 2643 (2018). doi.org/10.1038/s41467-018-05067-x
CRISPR-Based Chromatin Remodeling... doi.org/10.1016/j.stem.2017.12.001
Review article where figures are from doi.org/10.1152/physrev.00034.2019
Engineering cell fate - doi.org/10.1016/j.coisb.2020.09.002
Check out @LongevityScienceNews latest video - youtube.com/watch?v=I5IypXnjHok
Icons in intro; "freepik.com/free-photos-vectors/background"Background vector created by freepik - freepik.com
