The Sheekey Science Show | 3 advances in brain research (organoids, chips, microscopy) @TheSheekeyScienceShow | Uploaded September 2022 | Updated October 2024, 4 days ago.
Today we’re going to talk about a toolbox - a toolbox for brain research, looking at organs on a chip, organoids and microscopy and why you should be excited about them. Why should you care?
We still don’t know how to treat many different neurodegenerative diseases. We don’t fully understand the cause? We don’t know how personalised it is/how genetics affect things? Part of this has been due to a limitation in models and experimental techniques. We can’t just take biopsy of brain from healthy people, cell lines lack complexity and mouse models have been scrutinized as not representing the full process of disease manifestation resulting in poor translatability to human therapeutics.
So, in this video, i thought i would put my optimistic hat on, it’s almost always on, and provide you with an overview of the 3 novel methods being employed that may act as a better model for brains to advance neuroscience research.
Find me on Twitter - twitter.com/EleanorSheekey
Support the channel
through PayPal - paypal.me/sheekeyscience?country.x=GB&locale.x=en_GB
through Patreon - patreon.com/TheSheekeyScienceShow
TIMESTAMPS:
Intro - 00:00
Brain-on-chip - 01:50
Brain organoids - 03:40
Expansion microscopy - 06:40
References:
Generation of Functional Human 3D Cortico-Motor Assembloids - doi.org/10.1016/j.cell.2020.11.017
Pașca, S. The rise of three-dimensional human brain cultures. Nature 553, 437–445 (2018). doi.org/10.1038/nature25032
science.org/content/article/potential-fabrication-research-images-threatens-key-theory-alzheimers-disease
Leung, C.M., de Haan, P., Ronaldson-Bouchard, K. et al. A guide to the organ-on-a-chip. Nat Rev Methods Primers 2, 33 (2022). doi.org/10.1038/s43586-022-00118-6
Choi, S., Kim, Y., Hebisch, M. et al. A three-dimensional human neural cell culture model of Alzheimer’s disease. Nature 515, 274–278 (2014). doi.org/10.1038/nature13800
Expansion microscopy - doi.org/10.1126/science.1260088
Sarkar, D., Kang, J., Wassie, A.T. et al. Revealing nanostructures in brain tissue via protein decrowding by iterative expansion microscopy. Nat. Biomed. Eng (2022). doi.org/10.1038/s41551-022-00912-3
https://wyss.harvard.edu/technology/human-organs-on-chips/
emulatebio.com/brain-chip
Please note that The Sheekey Science Show is distinct from Eleanor Sheekey's teaching and research roles at the University of Cambridge. The information provided in this show is not medical advice, nor should it be taken or applied as a replacement for medical advice. The Sheekey Science Show and guests assume no liability for the application of the information discussed.
Icons in intro; "freepik.com/free-photos-vectors/background"Background vector created by freepik - freepik.com
Today we’re going to talk about a toolbox - a toolbox for brain research, looking at organs on a chip, organoids and microscopy and why you should be excited about them. Why should you care?
We still don’t know how to treat many different neurodegenerative diseases. We don’t fully understand the cause? We don’t know how personalised it is/how genetics affect things? Part of this has been due to a limitation in models and experimental techniques. We can’t just take biopsy of brain from healthy people, cell lines lack complexity and mouse models have been scrutinized as not representing the full process of disease manifestation resulting in poor translatability to human therapeutics.
So, in this video, i thought i would put my optimistic hat on, it’s almost always on, and provide you with an overview of the 3 novel methods being employed that may act as a better model for brains to advance neuroscience research.
Find me on Twitter - twitter.com/EleanorSheekey
Support the channel
through PayPal - paypal.me/sheekeyscience?country.x=GB&locale.x=en_GB
through Patreon - patreon.com/TheSheekeyScienceShow
TIMESTAMPS:
Intro - 00:00
Brain-on-chip - 01:50
Brain organoids - 03:40
Expansion microscopy - 06:40
References:
Generation of Functional Human 3D Cortico-Motor Assembloids - doi.org/10.1016/j.cell.2020.11.017
Pașca, S. The rise of three-dimensional human brain cultures. Nature 553, 437–445 (2018). doi.org/10.1038/nature25032
science.org/content/article/potential-fabrication-research-images-threatens-key-theory-alzheimers-disease
Leung, C.M., de Haan, P., Ronaldson-Bouchard, K. et al. A guide to the organ-on-a-chip. Nat Rev Methods Primers 2, 33 (2022). doi.org/10.1038/s43586-022-00118-6
Choi, S., Kim, Y., Hebisch, M. et al. A three-dimensional human neural cell culture model of Alzheimer’s disease. Nature 515, 274–278 (2014). doi.org/10.1038/nature13800
Expansion microscopy - doi.org/10.1126/science.1260088
Sarkar, D., Kang, J., Wassie, A.T. et al. Revealing nanostructures in brain tissue via protein decrowding by iterative expansion microscopy. Nat. Biomed. Eng (2022). doi.org/10.1038/s41551-022-00912-3
https://wyss.harvard.edu/technology/human-organs-on-chips/
emulatebio.com/brain-chip
Please note that The Sheekey Science Show is distinct from Eleanor Sheekey's teaching and research roles at the University of Cambridge. The information provided in this show is not medical advice, nor should it be taken or applied as a replacement for medical advice. The Sheekey Science Show and guests assume no liability for the application of the information discussed.
Icons in intro; "freepik.com/free-photos-vectors/background"Background vector created by freepik - freepik.com
