Uploaded October 2021 | Updated September 2026, 2 weeks ago

---

Learn how to properly dilute EveryBlot Block Buffer for faster western blot blocking and antibody incubation. EveryBlot Blocking Buffer is an optimized buffer for blocking membrane prior to immunodetection and for antibody dilution during western blotting. This blocker provides a 5-minute blocking time while maintaining maximum sensitivity for all western blots regardless of detection method. Features and Benefits;

- Reduces non-specific antibody binding to reduce background while maintaining excellent sensitivity
- For both chemiluminescent and fluorescent detection
- Contains no phosphate-based buffers. Ideal for use with phospho-specific antibodies
- Complete blocking in 5 minutes .

Learn more at bio-rad.com/QuickTips

Connect with Bio-Rad Online:
Visit Bio-Rad's Website: bio-rad.com
Follow Bio-Rad on LinkedIn: linkedin.com/company/bio-rad
Follow Bio-Rad on Facebook: facebook.com/biorad
Follow Bio-Rad on Instagram: instagram.com/bioradlabs

©2021 Bio-Rad is a trademark of Bio-Rad Laboratories, Inc. in certain jurisdictions. All trademarks used herein are the property of their respective owner.

Music used by permission granted by royalty-free license from Pond5.com

#westernblot #blockingbuffer #fluorescentwesternblotting