Uploaded June 2025 | Updated September 2026, 1 hour ago
PacBio Field Application Scientist Samantha Kalla details PureTarget, a powerful PCR-free method for targeted sequencing that preserves native methylation data. Learn how this CRISPR-Cas9-based approach enables the comprehensive analysis of challenging genomic regions, including GC-rich areas and repeat expansions, by enriching for targets directly from high-molecular-weight DNA.
The PCR-free workflow offers distinct advantages over traditional methods by retaining vital epigenetic information and improving data quality. Because it avoids amplification, PureTarget prevents GC dropout, minimizes size bias against long fragments, and eliminates PCR replication errors. This process results in highly accurate sequencing of original DNA molecules, which is especially critical for studying large, complex repeat expansion alleles. The workflow supports a sample-to-answer timeline of approximately three days, including sequencing and analysis.
Discover the comprehensive solution, featuring the PureTarget repeat expansion panel for 20 clinically relevant genes. When multiplexing up to 48 samples on a Revio or Vega system, the panel consistently yields high coverage. The Tandem Repeat Genotyping Tool (TRGT) supports downstream analysis, characterizing repeat motifs, allele lengths, and methylation status from HiFi reads.
00:00 Introducing PureTarget
00:16 The PCR-Free PureTarget Workflow
01:30 Key Advantages of PCR-Free Enrichment
02:55 Input DNA Quality Recommendations
03:45 Analyzing Data with the TRGT Tool
04:35 The PureTarget Repeat Expansion Panel
Subscribe for more insights from PacBio: youtube.com/@PacificBiosciences?sub_confirmation=1
Learn more about PacBio at pacb.com
Legal & Trademarks: Visit pacb.com/legal-and-trademarks
PacBio Field Application Scientist Samantha Kalla details PureTarget, a powerful PCR-free method for targeted sequencing that preserves native methylation data. Learn how this CRISPR-Cas9-based approach enables the comprehensive analysis of challenging genomic regions, including GC-rich areas and repeat expansions, by enriching for targets directly from high-molecular-weight DNA.
The PCR-free workflow offers distinct advantages over traditional methods by retaining vital epigenetic information and improving data quality. Because it avoids amplification, PureTarget prevents GC dropout, minimizes size bias against long fragments, and eliminates PCR replication errors. This process results in highly accurate sequencing of original DNA molecules, which is especially critical for studying large, complex repeat expansion alleles. The workflow supports a sample-to-answer timeline of approximately three days, including sequencing and analysis.
Discover the comprehensive solution, featuring the PureTarget repeat expansion panel for 20 clinically relevant genes. When multiplexing up to 48 samples on a Revio or Vega system, the panel consistently yields high coverage. The Tandem Repeat Genotyping Tool (TRGT) supports downstream analysis, characterizing repeat motifs, allele lengths, and methylation status from HiFi reads.
00:00 Introducing PureTarget
00:16 The PCR-Free PureTarget Workflow
01:30 Key Advantages of PCR-Free Enrichment
02:55 Input DNA Quality Recommendations
03:45 Analyzing Data with the TRGT Tool
04:35 The PureTarget Repeat Expansion Panel
Subscribe for more insights from PacBio: youtube.com/@PacificBiosciences?sub_confirmation=1
Learn more about PacBio at pacb.com
Legal & Trademarks: Visit pacb.com/legal-and-trademarks
