Uploaded April 2023 | Updated September 2026, 31 minutes ago

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STEMCELL’s portfolio of TeSR™ 3D-optimized media products have been developed for robust and scalable suspension culture of human pluripotent stem cells (hPSCs) in aggregates. The workflows include non-enzymatic dissociation using the Gentle Cell Dissociation Reagent and filter-based trituration. Watch this technical video to learn how to passage a 2 mL hPSC suspension culture in a 6-well plate.

Timecode:

0:00 Intro
0:26 Preparing required reagents and materials
1:42 Clump passaging a 3D hPSC culture using GCDR and filter-based trituration
5:09 Seeding hPSC clumps into a 6-well plate


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