Uploaded November 2020 | Updated September 2026, 2 hours ago

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This in-depth technical video demonstrates how to use SepMate™ tubes to isolate peripheral blood mononuclear cells (PBMCs) from whole blood or bone marrow in just 15 minutes.

0:00 Introduction
0:17 Protocol overview: PBMC isolation with standard density gradient with non- SepMate™ tubes vs isolation with SepMate™ tubes
0:32 SepMate™ tube features

Protocol walkthrough
1:46 What is needed for PBMC isolation with SepMate™?
2:33 Addition of density gradient media to the SepMate™ tube
4:10 Blood sample dilution
5:49 Addition of the diluted blood sample to the SepMate™ tube
7:07 Centrifugation of SepMate™ tube
8:50 Collection of isolated PBMCs
11:39 First wash of isolated PBMCs
13:55 Second wash of isolated PBMCs

16:01 Comparison of washed PBMC pellet after isolation with standard density gradient protocol with a non- SepMate™ tube

Learn more about SepMate™ PBMC isolation tubes at sepmate.com

#PBMCisolation #CellSeparation #CellIsolation

For a full list of products, as well as educational resources, visit our website: stemcell.com

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