Uploaded May 2025 | Updated September 2026, 5 hours ago
Explore targeted HiFi sequencing approaches with PacBio, offering a focused and cost-effective alternative to whole genome analysis for specific genes or regions. These methods enhance scalability and affordability, whether using the benchtop Vega system or the higher-throughput Revio system, providing robust coverage of difficult-to-sequence areas.
PacBio's targeted HiFi sequencing enables unambiguous, direct haplotype phasing without parental samples and delivers high-accuracy variant calling, including complex structural variants and copy number variations. This capability allows labs to consolidate multiple legacy workflows, such as Southern blotting or MLPA, into a more efficient, consistent NGS-based process, particularly valuable in clinical research and high-throughput environments. Discover three main approaches: PCR-free PureTarget for native DNA methylation and repeat expansion studies, long-read optimized HiFi target enrichment for comprehensive gene panels, and cost-effective amplicon sequencing for smaller panels or variant validation.
00:00 Introduction to Targeted HiFi Sequencing
00:20 Why Choose a Targeted Approach?
00:55 Advantages of Targeted HiFi Reads
01:36 Consolidating Legacy Molecular Workflows
02:21 Three Main Targeted HiFi Approaches
02:28 PureTarget: PCR-Free Enrichment
03:04 HiFi Target Enrichment Panels
03:39 Amplicon Sequencing for Targeted Regions
04:11 Choosing the Right Method
04:32 Benefits of Targeted HiFi Sequencing
Subscribe for more insights from PacBio: youtube.com/@PacificBiosciences?sub_confirmation=1
Learn more about PacBio at pacb.com
Legal & Trademarks: Visit pacb.com/legal-and-trademarks
Explore targeted HiFi sequencing approaches with PacBio, offering a focused and cost-effective alternative to whole genome analysis for specific genes or regions. These methods enhance scalability and affordability, whether using the benchtop Vega system or the higher-throughput Revio system, providing robust coverage of difficult-to-sequence areas.
PacBio's targeted HiFi sequencing enables unambiguous, direct haplotype phasing without parental samples and delivers high-accuracy variant calling, including complex structural variants and copy number variations. This capability allows labs to consolidate multiple legacy workflows, such as Southern blotting or MLPA, into a more efficient, consistent NGS-based process, particularly valuable in clinical research and high-throughput environments. Discover three main approaches: PCR-free PureTarget for native DNA methylation and repeat expansion studies, long-read optimized HiFi target enrichment for comprehensive gene panels, and cost-effective amplicon sequencing for smaller panels or variant validation.
00:00 Introduction to Targeted HiFi Sequencing
00:20 Why Choose a Targeted Approach?
00:55 Advantages of Targeted HiFi Reads
01:36 Consolidating Legacy Molecular Workflows
02:21 Three Main Targeted HiFi Approaches
02:28 PureTarget: PCR-Free Enrichment
03:04 HiFi Target Enrichment Panels
03:39 Amplicon Sequencing for Targeted Regions
04:11 Choosing the Right Method
04:32 Benefits of Targeted HiFi Sequencing
Subscribe for more insights from PacBio: youtube.com/@PacificBiosciences?sub_confirmation=1
Learn more about PacBio at pacb.com
Legal & Trademarks: Visit pacb.com/legal-and-trademarks
