Uploaded October 2025 | Updated September 2026, 1 day ago
*How can HiFi long-read sequencing achieve cost parity with short reads?* In this PacBio PRISM 2025 presentation, Mark Van Oene unveils the R&D roadmap for making high-throughput, low-cost HiFi sequencing a reality for large-scale genomics projects.
His work details the core innovations driving this change, including next-generation SMRT Cells from 300mm wafers, advanced SPRQ chemistry, and a novel multi-use SMRT Cell technology. These advancements are designed to dramatically reduce costs and enable projects involving tens of thousands of genomes per year.
*What you’ll learn*
* Explore next-generation SMRT Cell technology
* Understand advances in SPRQ chemistry
* Learn about multi-use SMRT Cell cost reductions
* See the future of targeted sequencing panels
*Featured speakers*
* Mark Van Oene, Chief Operating Officer, PacBio
00:00 The PacBio R&D vision
01:30 Future focus: The Revio and Vega systems, and high-throughput systems
02:55 The goal: Cost parity with short-read sequencing
04:24 SMRT Cell innovation: 300mm semiconductor wafers
06:10 Performance of new high-density SMRT Cells
07:28 Advancing SPRQ chemistry
10:04 The economics of multi-use SMRT Cells
11:10 Proof of concept: Reusing SMRT Cells up to four times
14:16 Advancements in computational analysis pipelines
16:29 New epigenetic calling with the HK2 model
17:35 Expanding applications for targeted panels
19:33 Summary: The path to accessible HiFi sequencing
What large-scale project would you start if HiFi sequencing cost $200 per genome? *Comment below.*
*Subscribe for more PacBio genomics content:*
youtube.com/@PacificBiosciences?sub_confirmation=1
Learn more about PacBio at pacb.com
Legal & Trademarks: pacb.com/legal-and-trademarks
#HiFiSequencing #Genomics #PacBio
*How can HiFi long-read sequencing achieve cost parity with short reads?* In this PacBio PRISM 2025 presentation, Mark Van Oene unveils the R&D roadmap for making high-throughput, low-cost HiFi sequencing a reality for large-scale genomics projects.
His work details the core innovations driving this change, including next-generation SMRT Cells from 300mm wafers, advanced SPRQ chemistry, and a novel multi-use SMRT Cell technology. These advancements are designed to dramatically reduce costs and enable projects involving tens of thousands of genomes per year.
*What you’ll learn*
* Explore next-generation SMRT Cell technology
* Understand advances in SPRQ chemistry
* Learn about multi-use SMRT Cell cost reductions
* See the future of targeted sequencing panels
*Featured speakers*
* Mark Van Oene, Chief Operating Officer, PacBio
00:00 The PacBio R&D vision
01:30 Future focus: The Revio and Vega systems, and high-throughput systems
02:55 The goal: Cost parity with short-read sequencing
04:24 SMRT Cell innovation: 300mm semiconductor wafers
06:10 Performance of new high-density SMRT Cells
07:28 Advancing SPRQ chemistry
10:04 The economics of multi-use SMRT Cells
11:10 Proof of concept: Reusing SMRT Cells up to four times
14:16 Advancements in computational analysis pipelines
16:29 New epigenetic calling with the HK2 model
17:35 Expanding applications for targeted panels
19:33 Summary: The path to accessible HiFi sequencing
What large-scale project would you start if HiFi sequencing cost $200 per genome? *Comment below.*
*Subscribe for more PacBio genomics content:*
youtube.com/@PacificBiosciences?sub_confirmation=1
Learn more about PacBio at pacb.com
Legal & Trademarks: pacb.com/legal-and-trademarks
#HiFiSequencing #Genomics #PacBio
