Library preparation and HiFi sequencing applications for AAV @PacificBiosciences

Uploaded September 2023 | Updated September 2026, 8 hours ago

In this webinar, Edward Oakeley, PhD, Novartis, shares and explains his experience and the developments of the HiFi AAV genome sequencing method over time and how his group has developed a modified library preparation protocol to maximize the usefulness of PacBio HiFi reads for AAV characterization. The modified library prep protocol includes adding an additional step for the synthesis of a complementary second strand in order to capture a greater variety of AAV molecules in the analysis. Gain a deep understanding of the unique advantages that HiFi sequencing brings to the table, empowering you to make informed decisions that can significantly impact the safety and efficacy of gene therapy products.

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