Uploaded March 2026 | Updated September 2026, 1 day ago

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HiFi sequencing can read DNA methylation and chromatin accessibility directly from native DNA — no bisulfite conversion needed. Two cancer cases show why that matters.

In episode 2 of Cancer Sequencing 101, Samantha Kalla explains how HiFi long-read sequencing detects methylation through polymerase kinetics — delivering that data with every run, essentially for free. She also introduces Fiber-seq, an assay that maps which regions of chromatin are open and accessible.

Explore the interactive cancer body map: programs.pacb.com/l/1652/2026-03-10/45fw1y

*What you'll learn*
- How HiFi detects methylation natively — no bisulfite conversion required
- How to phase methylation with genetic variants on the same long read
- What Fiber-seq reveals about chromatin accessibility in cancer
- How 2 real cancer cases cracked tumor suppressor silencing

*Featured speakers*
- Samantha Kalla, Field Application Scientist (PacBio)

*Chapters*
00:00 Welcome back to Cancer Sequencing 101
00:10 DNA methylation in cancer
00:55 The problem with bisulfite conversion
01:26 How HiFi detects methylation natively
02:19 Sarcoma case: BRCA2 two-hit inactivation
03:05 Chromatin accessibility and Fiber-seq
04:31 Retinoblastoma case: RB1 silencing
05:11 Explore the cancer body map

*Resources*
- Interactive cancer body map: programs.pacb.com/l/1652/2026-03-10/45fw1y
- PacBio cancer research: pacb.com/cancer-research
- PacBio Epigenetics: pacb.com/products-and-services/applications/epigenetics

Have you worked with epigenetic data? What surprised you? *Comment below.*

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#CancerGenomics #Epigenetics #HiFiSequencing