Uploaded March 2025 | Updated September 2026, 3 weeks ago

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This video shows how to use Sample Purification Beads (SPB) & Illumina Purification Beads (IPB) to purify cDNA or gDNA fragments and remove unwanted products and reaction components.

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00:00 Introduction
00:08 Vortex SPB beads and add to a MIDI plate with samples
01:16 Incubation steps including magnetic plate
01:38 Tips for sample placement and pipetting
02:00 Remove supernatant from wells
02:52 Wash beads on magnetic stand with 80% ethanol
04:04 Tips for best magnetic stand bead visibility
04:37 Add Resuspension Buffer (RSB) to wells over bead pellet – Method 1 – single channel pipette
05:21 Add Resuspension Buffer (RSB) to wells over bead pellet – Method 2 – multichannel with strip tube
06:24 Incubation steps
06:34 Transfer eluate to new PCR plate