Uploaded September 2020 | Updated September 2026, 1 day ago

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In this series of videos, we will guide you through the protocols for expanding and differentiating human bronchial epithelial cells (HBECs) at the air-liquid interface (ALI). We will use the PneumaCult™ culture system to demonstrate ALI culture for this video. If you are using other culture media, some alterations to this protocol may be necessary.

00:00 Introduction
00:48 Overview of the ALI culture workflow using PneumaCult™
01:02 Overview of phase 1 - Expansion culture in a flask
01:15 Overview of phase 2 - Submerged expansion in porous inserts
01:37 Overview of phase 3 - ALI culture differentiation in inserts

Watch the other videos in this series:
Video 1: How to Model the Human Airway at the ALI: Expansion of HBECs - youtu.be/ovjjPPlWFjQ
Video 2: How to Model the Human Airway at the Air-Liquid Interface: Differentiation of HBECs - youtu.be/MbXwvBWQv0A

To learn more about PneumaCult™, visit us at pneumacult.com

#alicultures #airliquidinterface #pneumacult #airwayresearch

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