Uploaded September 2020 | Updated September 2026, 4 days ago

---

In this protocol video, you will learn how to differentiate human bronchial epithelial cells (HBECs) at the air-liquid interface (ALI) using PneumaCult™ culture media.

00:00 Introduction
00:28 Media preparation
01:46 Air-lifting the culture
03:11 Time course of morphological changes in culture
03:50 Histological staining

Watch the other videos in this series:

How to Model the Human Airway at the Air-Liquid Interface: Introduction - youtu.be/u2OLfPT8qIw
How to Model the Human Airway at the Air-Liquid Interface: Expansion of HBECs - youtu.be/ovjjPPlWFjQ

For our full list of products visit our website stemcell.com

Follow STEMCELL Technologies on Twitter at twitter.com/STEMCELLTech
Like STEMCELL Technologies on Facebook at facebook.com/stemcelltechnologies
Subscribe to STEMCELL Technologies on YouTube at youtube.com/STEMCELLTechnologies