Uploaded July 2023 | Updated September 2026, 2 weeks ago
Digital PCR (dPCR) can provide absolute quantification, without standard curves, along with unrivaled accuracy and precision. With more dPCR providers than every in the marketplace these is a lot of talk about which dPCR system has the lowest dead volume, which matters when there is limited sample volume and/or low copy number target in the sample.
Dead volume is the portion of the sample that’s not directly used in the analytical analysis. Volume can be lost in collection, preparation, and in the dPCR analysis process itself. Essentially, the entire dPCR workflow needs to be considered when discussing dead volume.
The early dPCR workflow steps of sample collection and sample preparation are often neglected but should not be as sample volume and target template copy number can easily be lost here. Optimization of these steps should be considered, regardless of dPCR platform being used.
In the dPCR portion of the workflow every provider partitions the sample, amplifies the targets, and then reads and analyzes the sample. However, Bio-Rad™ Droplet Digital™ PCR (ddPCR™) does things differently. Specifically, only ddPCR technology uses emulsion-based partitioning and only ddPCR technology offers gradient capabilities in the PCR amplification step. Droplet partitioning creates up to 20,000 sample partitions, regardless of consumable choices, to ensure that under sampling errors are avoided. The gradient capabilities of ddPCR technology allow for faster PCR optimization to minimize sample used during optimizations steps, a consideration often overlooked by others.
Digital PCR dead volume is important in situations with limited sample and applications with rare targets. For these situations consider the entire dPCR workflow to optimize early steps and select a dPCR platform that reliably makes the most of your precious samples. Choose ddPCR technology info.bio-rad.com/what-difference-d-makes.html?WT.mc_id=230726038531!
#digitalPCR #ddPCR #dPCR #deadvolume #workflow #optimization
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Website: bio-rad.com/digital-pcr
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©2023 Bio-Rad is a trademark of Bio-Rad Laboratories, Inc. in certain jurisdictions. All trademarks used herein are the property of their respective owner.
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Digital PCR (dPCR) can provide absolute quantification, without standard curves, along with unrivaled accuracy and precision. With more dPCR providers than every in the marketplace these is a lot of talk about which dPCR system has the lowest dead volume, which matters when there is limited sample volume and/or low copy number target in the sample.
Dead volume is the portion of the sample that’s not directly used in the analytical analysis. Volume can be lost in collection, preparation, and in the dPCR analysis process itself. Essentially, the entire dPCR workflow needs to be considered when discussing dead volume.
The early dPCR workflow steps of sample collection and sample preparation are often neglected but should not be as sample volume and target template copy number can easily be lost here. Optimization of these steps should be considered, regardless of dPCR platform being used.
In the dPCR portion of the workflow every provider partitions the sample, amplifies the targets, and then reads and analyzes the sample. However, Bio-Rad™ Droplet Digital™ PCR (ddPCR™) does things differently. Specifically, only ddPCR technology uses emulsion-based partitioning and only ddPCR technology offers gradient capabilities in the PCR amplification step. Droplet partitioning creates up to 20,000 sample partitions, regardless of consumable choices, to ensure that under sampling errors are avoided. The gradient capabilities of ddPCR technology allow for faster PCR optimization to minimize sample used during optimizations steps, a consideration often overlooked by others.
Digital PCR dead volume is important in situations with limited sample and applications with rare targets. For these situations consider the entire dPCR workflow to optimize early steps and select a dPCR platform that reliably makes the most of your precious samples. Choose ddPCR technology info.bio-rad.com/what-difference-d-makes.html?WT.mc_id=230726038531!
#digitalPCR #ddPCR #dPCR #deadvolume #workflow #optimization
Subscribe to the Bio-Rad channel: youtube.com/user/BioRadLifeScience?sub_confirmation=1
We Are Bio-Rad.
Our mission: To provide useful, high-quality products and services that advance scientific discovery and improve healthcare. At Bio-Rad, we are united behind this effort. These two objectives are the driving force behind every decision we make, from developing innovative ideas to building global solutions that help solve our customers' greatest challenges.
Connect with Bio-Rad Online:
Website: bio-rad.com/digital-pcr
LinkedIn: linkedin.com/company/bio-rad
Facebook: facebook.com/BioRadGenomics
Twitter: twitter.com/BioRadGenomics
Instagram: instagram.com/bioradlabs
©2023 Bio-Rad is a trademark of Bio-Rad Laboratories, Inc. in certain jurisdictions. All trademarks used herein are the property of their respective owner.
Music used by permission granted by royalty-free license from Pond5.com
