Uploaded June 2022 | Updated September 2026, 2 weeks ago
Monoclonal antibody (mAb) purification methods often involve the use of resin-based chromatography in a series of steps. A typical mAb purification process involves a multi-step workflow consisting of two or more columns for capture, intermediate, and polish purification to isolate the target molecule. Resins selected for each of these steps solve specific challenges that exist at that phase of purification for the mAb. Purification strategies with combined mechanisms of action can allow for fewer chromatography steps to save processing time, thereby making enhancements to overall process economics and efficiency. We compare a three-step mAb purification using Nuvia Ion Exchange Resins post Protein A capture, to a two-step purification workflow with a Protein A capture step, followed by a mixed-mode chromatography step using Nuvia aPrime 4A, a hydrophobic anion exchange resin. We will present an additional two-step purification approach using Protein A Resin followed by a calcium affinity cation exchange media, CHT Ceramic Hydroxyapatite XT. The results of both studies will show the ability to achieve high purity product with one fewer unit operation.
Learn more at bio-rad.com/en-us/process-separations/support/webinars?ID=KVY6W0MNI
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#chromatography #purification #vaccine
Our mission: To provide useful, high-quality products and services that advance scientific discovery and improve healthcare. At Bio-Rad, we are united behind this effort. These two objectives are the driving force behind every decision we make, from developing innovative ideas to building global solutions that help solve our customers' greatest challenges.
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©2022 Bio-Rad is a trademark of Bio-Rad Laboratories, Inc. in certain jurisdictions. All trademarks used herein are the property of their respective owner.
Music used by permission granted by royalty-free license from Pond5.com
Monoclonal antibody (mAb) purification methods often involve the use of resin-based chromatography in a series of steps. A typical mAb purification process involves a multi-step workflow consisting of two or more columns for capture, intermediate, and polish purification to isolate the target molecule. Resins selected for each of these steps solve specific challenges that exist at that phase of purification for the mAb. Purification strategies with combined mechanisms of action can allow for fewer chromatography steps to save processing time, thereby making enhancements to overall process economics and efficiency. We compare a three-step mAb purification using Nuvia Ion Exchange Resins post Protein A capture, to a two-step purification workflow with a Protein A capture step, followed by a mixed-mode chromatography step using Nuvia aPrime 4A, a hydrophobic anion exchange resin. We will present an additional two-step purification approach using Protein A Resin followed by a calcium affinity cation exchange media, CHT Ceramic Hydroxyapatite XT. The results of both studies will show the ability to achieve high purity product with one fewer unit operation.
Learn more at bio-rad.com/en-us/process-separations/support/webinars?ID=KVY6W0MNI
Subscribe to the Bio-Rad channel: youtube.com/user/BioRadLifeScience?sub_confirmation=1
We Are Bio-Rad.
#chromatography #purification #vaccine
Our mission: To provide useful, high-quality products and services that advance scientific discovery and improve healthcare. At Bio-Rad, we are united behind this effort. These two objectives are the driving force behind every decision we make, from developing innovative ideas to building global solutions that help solve our customers' greatest challenges.
Connect with Bio-Rad Online:
Website: bio-rad.com
LinkedIn: linkedin.com/company/1613226
Facebook: facebook.com/biorad
Twitter: twitter.com/BioRadLifeSci
Instagram: @BioRadLabs
Snapchat: @BioRadLabs
©2022 Bio-Rad is a trademark of Bio-Rad Laboratories, Inc. in certain jurisdictions. All trademarks used herein are the property of their respective owner.
Music used by permission granted by royalty-free license from Pond5.com
