Uploaded October 2024 | Updated September 2026, 1 hour ago
Seminar Title: Investigation of RNA modifications associated with glucose stimulation in pancreatic islet β-cells
September 26, 2024 - Clinical and genetic studies highlight the central pathophysiological role of pancreatic islet β-cell dysfunction in Type 2 diabetes (T2D). However, the molecular mechanisms driving T2D in pancreatic islet β-cells remains unclear. Recent studies suggest an important role for N-6-methyladenosine (m6A) in T2D. Largely due to technical detection challenges, the impact that any other RNA modifications have not been investigated in the context of T2D. Recent advances in direct RNA nanopore sequencing (dRNA-seq) allow for direct detection of all all RNA modifications simultaneously. Here we sequenced poly(A) seleteced RNA from the human β-cell line, EndoC-bH3 grown at basal (2.8mM) and stimulated (15mM) glucose conditions. With an average of 17M reads from four replicates of each condition, we found 2643 m6A and 1522 pseudouridine RNA modifications that were significantly different after glucose stimulation. Among these, 63 m6A and 26 pseudouridines were found on transcripts previously associated with T2D. We aim to use these results to guide future large-scale studies of RNA modifications in the context of T2D.
Relevant Links:
Logan Mulroney: https://www.iit.it/people-details/-/people/logan-mulroney
About the Seminar: genome.gov/cphr/seminars
Seminar Title: Investigation of RNA modifications associated with glucose stimulation in pancreatic islet β-cells
September 26, 2024 - Clinical and genetic studies highlight the central pathophysiological role of pancreatic islet β-cell dysfunction in Type 2 diabetes (T2D). However, the molecular mechanisms driving T2D in pancreatic islet β-cells remains unclear. Recent studies suggest an important role for N-6-methyladenosine (m6A) in T2D. Largely due to technical detection challenges, the impact that any other RNA modifications have not been investigated in the context of T2D. Recent advances in direct RNA nanopore sequencing (dRNA-seq) allow for direct detection of all all RNA modifications simultaneously. Here we sequenced poly(A) seleteced RNA from the human β-cell line, EndoC-bH3 grown at basal (2.8mM) and stimulated (15mM) glucose conditions. With an average of 17M reads from four replicates of each condition, we found 2643 m6A and 1522 pseudouridine RNA modifications that were significantly different after glucose stimulation. Among these, 63 m6A and 26 pseudouridines were found on transcripts previously associated with T2D. We aim to use these results to guide future large-scale studies of RNA modifications in the context of T2D.
Relevant Links:
Logan Mulroney: https://www.iit.it/people-details/-/people/logan-mulroney
About the Seminar: genome.gov/cphr/seminars
