Beckman Institute at Illinois | Cold as (vitreous) ice: high pressure freezing microscopic worms @BeckmanIllinois | Uploaded June 2021 | Updated October 2024, 2 hours ago.
In normal microscope preps, ice crystals that form as a sample is frozen can damage the cell's ultrastructure and create a less-than-perfect image. Microscopy Suite Director Scott Robinson shows how using high pressure freezing/freeze substitution provides unparalleled cellular preservation. This is an abbreviated version of the longer video here youtu.be/Stf-TUfdHrc