Uploaded January 2022 | Updated September 2026, 1 day ago

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Dr. Nastassia Patin, a postdoctoral associate with the National Oceanic and Atmospheric Administration’s Atlantic and Oceanographic Meteorological Laboratory (NOAA AOML), provides a presentation on the basics of analyzing shotgun metagenomes, specifically short read metagenomes. Nastassia Patin can be contacted at nastassia.patin@noaa.gov.

Timestamps:
00:00 - Introduction
00:51 - General workflow
02:02 - 01. Read quality control
04:15 - 02. Read-based analyses
09:28 - 03. Assembly
11:48 - 04. Gene prediction
13:00 - 05. Gene annotation
17:35 - 06. Coverage
19:35 - 07. Binning
23:53 - 08. Genome quality
25:12 - 09. Genome taxonomy
26:17 - 10. Dereplicating MAGs
29:28 - Summary
32:02 - Pan-metagenome thought exercise