Uploaded July 2023 | Updated September 2026, 2 days ago
Chris Masterjohn, PhD is the Founder and Scientific Director of the mitochondrial test mito.me. Question: What cofactors are needed to synthesize and recycle BH4?
Short Answer: Zinc, magnesium, potassium, and niacin are the cofactors needed for the synthesis and recycling of BH4. Folate and methylation are not involved, though high-dose folate or folic acid could hypothetically hurt BH4 recycling since both are recycled by dihydrofolate reductase (DHFR).
This is a clip from a live Q&A session open to CMJ Masterpass members. In addition to this episode, you can access two other free samples using this link:
chrismasterjohnphd.substack.com/p/questions-on-vitamin-d-sulfate-synthesis
In that batch of free episodes you will also find the answers to these questions:
*Is It Important to Get Vitamin D Sulfate Specifically From the Sun?
*How Much Iron Can We Absorb At Once?
If you want to become a Masterpass member so you can participate in the next live Q&A, or so you can have access to the complete recording and transcript of each Q&A session, you can save 10% off the subscription price for as long as you remain a member by using this link to sign up:
chrismasterjohnphd.substack.com/qanda
Learn more about the Masterpass here:
chrismasterjohnphd.substack.com/about
This snippet is from the February 15, 2023 AMA. The full recording and transcript is reserved for Masterpass members. Here is a preview of what’s included:
*Is It Important to Get Vitamin D Sulfate Specifically From the Sun?
*How Much Iron Can We Absorb At Once?
*What nutrients are important for long-term PPI use?
*For how long does transferrin saturation respond to recent iron-rich food?
*Muscle spasms: creatine, creatinine, sodium, and potassium.
*Hematologists ignore iron saturation.
*How to detox arsenic?
*Could folic acid supplements impair BH4 recycling?
*How to increase butyrate?
*More on hematologists and transferrin saturation.
Here’s a link to the full AMA: chrismasterjohnphd.substack.com/p/recording-and-transcript-of-the-february
For more information, visit chrismasterjohn-phd.com
Do You Have Hidden Mitochondrial Dysfunction?
Mitochondrial dysfunction is one of the earliest signs of aging and chronic disease—and most people don’t know they have it. Mitome is the first at-home test that measures your cellular energy directly and gives you a personalized roadmap to optimize energy, slow aging, and protect against disease.
Find my comprehensive mitochondrial analysis at mito.me
Chris Masterjohn, PhD is the Founder and Scientific Director of the mitochondrial test mito.me. Question: What cofactors are needed to synthesize and recycle BH4?
Short Answer: Zinc, magnesium, potassium, and niacin are the cofactors needed for the synthesis and recycling of BH4. Folate and methylation are not involved, though high-dose folate or folic acid could hypothetically hurt BH4 recycling since both are recycled by dihydrofolate reductase (DHFR).
This is a clip from a live Q&A session open to CMJ Masterpass members. In addition to this episode, you can access two other free samples using this link:
chrismasterjohnphd.substack.com/p/questions-on-vitamin-d-sulfate-synthesis
In that batch of free episodes you will also find the answers to these questions:
*Is It Important to Get Vitamin D Sulfate Specifically From the Sun?
*How Much Iron Can We Absorb At Once?
If you want to become a Masterpass member so you can participate in the next live Q&A, or so you can have access to the complete recording and transcript of each Q&A session, you can save 10% off the subscription price for as long as you remain a member by using this link to sign up:
chrismasterjohnphd.substack.com/qanda
Learn more about the Masterpass here:
chrismasterjohnphd.substack.com/about
This snippet is from the February 15, 2023 AMA. The full recording and transcript is reserved for Masterpass members. Here is a preview of what’s included:
*Is It Important to Get Vitamin D Sulfate Specifically From the Sun?
*How Much Iron Can We Absorb At Once?
*What nutrients are important for long-term PPI use?
*For how long does transferrin saturation respond to recent iron-rich food?
*Muscle spasms: creatine, creatinine, sodium, and potassium.
*Hematologists ignore iron saturation.
*How to detox arsenic?
*Could folic acid supplements impair BH4 recycling?
*How to increase butyrate?
*More on hematologists and transferrin saturation.
Here’s a link to the full AMA: chrismasterjohnphd.substack.com/p/recording-and-transcript-of-the-february
For more information, visit chrismasterjohn-phd.com
Do You Have Hidden Mitochondrial Dysfunction?
Mitochondrial dysfunction is one of the earliest signs of aging and chronic disease—and most people don’t know they have it. Mitome is the first at-home test that measures your cellular energy directly and gives you a personalized roadmap to optimize energy, slow aging, and protect against disease.
Find my comprehensive mitochondrial analysis at mito.me
