Nigel Baldwin | TLC of food colours C0056 @nigelbaldwin3184 | Uploaded April 2020 | Updated October 2024, 5 hours ago.
High School Chemistry
Silica thin layer chromatography, TLC, on food dyes.
Small samples of pink, violet, red and blue food colours were spotted onto a 40x80mm Polygram silica gel 60 TLC plate. The base line onto which the samples were spotted was drawn 10mm from the bottom of the plate using a pencil.
The plate was eluted in a solvent mixture consisting of propan-2-ol 80% and water 20% by volume.
The TLC analysis conformed that the violet colour was a mixture of red and blue food colours and contained no pink colour.
Pink = Erythrosine, Red = Carmoisine and Blue = Brilliant Blue, according to the information on the labels.
High School Chemistry
Silica thin layer chromatography, TLC, on food dyes.
Small samples of pink, violet, red and blue food colours were spotted onto a 40x80mm Polygram silica gel 60 TLC plate. The base line onto which the samples were spotted was drawn 10mm from the bottom of the plate using a pencil.
The plate was eluted in a solvent mixture consisting of propan-2-ol 80% and water 20% by volume.
The TLC analysis conformed that the violet colour was a mixture of red and blue food colours and contained no pink colour.
Pink = Erythrosine, Red = Carmoisine and Blue = Brilliant Blue, according to the information on the labels.
