The Mammalian Nervous System featuring Dr. Freda Miller | The Stem Cell Podcast @STEMCELLTechnologies

Uploaded June 2020 | Updated September 2026, 2 days ago

The Stem Cell Podcast | Episode 169
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Culturing knowledge in stem cell research
The Mammalian Nervous System featuring Dr. Freda Miller

00:00 Intro
01:40 Episode intro
36:26 Interview with Dr. Freda Miller

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$How to Isolate Human Regulatory T Cells (Tregs) Using the Easy 50 EasySep™ Magnet This video is a step by step demonstration of how to isolate human regulatory T cells starting from up to 2 billion (2 x 10^9) peripheral blood mononuclear cells (PBMCs) or leukapheresis cells with the EasySep™ Human CD4+CD127lowCD25+ Regulatory T Cell Isolation Kit (Catalog # 18063) and the Easy 50 EasySep™ Magnet (Catalog # 18002). Please also consult the Product Information Sheet for further details on the protocol. EasySep™ Human CD4+CD127lowCD25+ Regulatory T Cell Isolation Kit (Catalog# 18063): https://bit.ly/3aejV9v Easy 50 EasySep™ Magnet (Catalog # 18002): https://bit.ly/3mLBra2 Product Information Sheet: https://bit.ly/2QqM9H3 00:00 Intro 04:04 Part 1: Isolation of CD25+ cells 04:15 Addition of the EasySep™ Human CD25 Positive Selection Cocktail (Catalog# 18063C.1) 06:15 Addition of the EasySep™ Releasable RapidSpheres™ 50201 and the EasySep™ Human CD4+ T Cell Enrichment Cocktail (Catalog# 19052C.2) 09:04 1st magnetic separation 11:52 Addition of EasySep™ Releasable RapidSpheres™ 50201 to supernatant collected after 1st magnetic separation 14:36 Transferring supernatant back to tube seated in magnet 15:13 2nd magnetic separation 16:26 Saving supernatant for optional isolation of CD4+CD25- responder T cells 16:37 3rd magnetic separation with wash step of CD25+ cells bound to EasySep™ Releasable RapidSpheres™ 50201 18:22 4th magnetic separation with wash step of CD25+ cells bound to EasySep™ Releasable RapidSpheres™ 50201 21:37 Sample resuspension + addition of Release Buffer 25:14 Part 2: Isolation of CD4+CD127low cells from CD25+ cell fraction 25:49 Addition of EasySep™ Human CD127high Depletion Cocktail (Catalog# 19233C) 27:05 Addition of the EasySep™ Dextran RapidSpheres™ 50100 29:06 Magnetic separation of the CD4+CD127lowCD25+ regulatory T cells 31:24 Optional Part: Isolation of CD4+CD25- responder T cells #Treg #RegulatoryTcell #CellSeparation #CellIsolation #EasySep For a full list of products, as well as educational resources, visit our website: https://www.stemcell.com Follow us on Twitter: https://bit.ly/3jW20Gv Like us on Facebook: https://bit.ly/32ern07 Follow us on LinkedIn: https://bit.ly/32uKovJ Subscribe to our YouTube channel: https://bit.ly/32f28ei$

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